Potential antigen-presenting cells in normal extraocular muscles demonstrated with double immunoenzyme staining
| Autor: | E. D. Schmidt, Pranab K. Das, Leo Koornneef, R van der Gaag, C. M. van Der Loos, A. J. Tigges |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Aged
80 and over Pathology medicine.medical_specialty Macrophages Muscles Monocyte Antigen-Presenting Cells Connective tissue Cell Count HLA-DR Antigens Biology Extraocular muscles Pathology and Forensic Medicine Immunoenzyme Techniques medicine.anatomical_structure Immune system Antigen medicine Humans Immunohistochemistry Macrophage Antigen-presenting cell Orbit Aged |
| Zdroj: | Journal of pathology, 164(2), 135-143. John Wiley and Sons Ltd |
| ISSN: | 0022-3417 |
| DOI: | 10.1002/path.1711640207 |
| Popis: | Antigen-presenting cells are of crucial importance for the initiation and regulation of regional immune responses. In a previous study, indirect morphological evidence that morphologically normal human orbital tissues contain HLA-DR-positive macrophages, which may represent antigen-presenting cells, has ben obtained. In the present study, these cells were characterized in detail using double immunoenzyme staining techniques with monoclonal antibodies directed against several well-characterized monocyte/macrophage markers and against HLA-DR gene products. The orbital muscular tissues appear to contain numerous HLA-DR, monocyte/macrophage marker double-stained cells, which are considered to be potential antigen-presenting cells. The cells are widely distributed in the connective tissue of all the orbital muscular tissues studied and consist of several subsets with different phenotypes. Furthermore, site-specific differences were shown between recti muscles and the levator/Müller's muscles with respect to the distribution of HLA-DR and one monocyte/macrophage marker (OKM5). Many of the orbital antigen-presenting cells appear to be of the dendritic type and are considered to be of major importance in regulating local orbital immunity. |
| Databáze: | OpenAIRE |
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