Comparison of the inhibitory effects of procyanidins with different structures and their digestion products against acrylamide-induced cytotoxicity in IPEC-J2 cells
| Autor: | Fangfang Yan, Cuie Tang, Rui Liu, Qun Lu, Chengming Wang, Wanbing Chen, Li Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology MTT assay TX341-641 Food science Procyanidin B3 Cytotoxicity Procyanidins Acrylamide 030109 nutrition & dietetics Nutrition and Dietetics Chemistry Nutrition. Foods and food supply In vitro digestion Catechin 04 agricultural and veterinary sciences Glutathione 040401 food science IPEC-J2 cells Digestion Procyanidin A1 (Epi)catechin Food Science |
| Zdroj: | Journal of Functional Foods, Vol 72, Iss, Pp 104073-(2020) |
| ISSN: | 1756-4646 |
| Popis: | This study evaluated the stabilities of catechin (C), epicatechin (EC), procyanidin B3, procyanidin A1 and epicatechin-(4β → 6)-epicatechin-(2β → O → 7,4β → 8)-catechin (PCA-trimer) during in vitro digestion, as well as characterized their digestion products (DPs), and evaluated their protective effects against ACR-induced cytotoxicity on IPEC-J2 cells. The results showed that procyanidin A1 (70.38%) and procyanidin B3 (15.25%) had the highest and lowest stability in the gastric phase, respectively. During the intestinal phase, the contents of C, EC and procyanidin B3 increased from 57.37%, 49.92% and 15.25% to 88.15%, 71.81% and 46.72%, while those of procyanidin A1 and PCA-trimer showed continuous decreases to 27.13% and 28.89%, respectively. Isomerism, hydrolysis and condensation reactions were found to play major roles in in vitro digestion. Besides, procyanidin A1 and its DPs exhibited the best protective effect against ACR-induced cytotoxicity compared with other compounds and their DPs, as indicated by MTT assay and measurement of glutathione (GSH). |
| Databáze: | OpenAIRE |
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