B‐cell profile, B‐cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
Autor: | Susana A. Laucella, María Cecilia Albareda, Noel Badano, Alejandra Barrio, Julia Pimentel, Ana G. González Prieto, Maria M.E. de Bracco, Melisa E. Castro Eiro, Cecilia Parodi, María F. García Bustos |
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Rok vydání: | 2020 |
Předmět: |
Adult
Leishmaniasis Mucocutaneous Male 0301 basic medicine Adolescent 030231 tropical medicine Immunology Leishmaniasis Cutaneous Flow cytometry Young Adult 03 medical and health sciences 0302 clinical medicine Cutaneous leishmaniasis B-Cell Activating Factor medicine Humans Interferon gamma B-cell activating factor Receptor Interleukin 6 B cell Aged B-Lymphocytes medicine.diagnostic_test biology Hypergammaglobulinemia Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure Immunoglobulin G biology.protein Cytokines Female Parasitology B-Cell Activation Factor Receptor medicine.drug |
Zdroj: | Parasite Immunology. 42 |
ISSN: | 1365-3024 0141-9838 |
Popis: | AIMS The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral-nasal-pharyngeal cavities. METHODS AND RESULTS By flow cytometry analysis, we found decreased percentages of non-class-switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B-cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity. Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN-γ and IL-6 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL. CONCLUSION We demonstrate B-cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML. |
Databáze: | OpenAIRE |
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