The Marine Fungal Metabolite, AD0157, Inhibits Angiogenesis by Targeting the Akt Signaling Pathway
| Autor: | Antonio Fernández-Medarde, Melissa García-Caballero, Ana R. Quesada, Miguel Ángel Medina, Librada Cañedo |
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| Rok vydání: | 2014 |
| Předmět: |
pyrrolidinedione
Embryo Nonmammalian MAP Kinase Signaling System Angiogenesis Drug Evaluation Preclinical Succinimides Pharmaceutical Science Angiogenesis Inhibitors DNA Fragmentation Biology Article Neovascularization Mice angiogenesis In vivo Cell Line Tumor Drug Discovery medicine Animals Humans AD0157 cancer Phosphorylation lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) Zebrafish Dose-Response Relationship Drug Akt/PKB signaling pathway Quinones apoptosis Endothelial Cells marine drug Chromatin Capillaries Extracellular Matrix Cell biology Enzyme Activation Oncogene Protein v-akt Chorioallantoic membrane lcsh:Biology (General) Cell culture Caspases DNA fragmentation medicine.symptom Ex vivo Signal Transduction |
| Zdroj: | Marine Drugs Volume 12 Issue 1 Pages: 279-299 Marine Drugs, Vol 12, Iss 1, Pp 279-299 (2014) |
| ISSN: | 1660-3397 |
| Popis: | In the course of a screening program for the inhibitors of angiogenesis from marine sources, AD0157, a pyrrolidinedione fungal metabolite, was selected for its angiosupressive properties. AD0157 inhibited the growth of endothelial and tumor cells in culture in the micromolar range. Our results show that subtoxic doses of this compound inhibit certain functions of endothelial cells, namely, differentiation, migration and proteolytic capability. Inhibition of the mentioned essential steps of in vitro angiogenesis is in agreement with the observed antiangiogenic activity, substantiated by using two in vivo angiogenesis models, the chorioallantoic membrane and the zebrafish embryo neovascularization assays, and by the ex vivo mouse aortic ring assay. Our data indicate that AD0157 induces apoptosis in endothelial cells through chromatin condensation, DNA fragmentation, increases in the subG1 peak and caspase activation. The data shown here altogether indicate for the first time that AD0157 displays antiangiogenic effects, both in vitro and in vivo, that are exerted partly by targeting the Akt signaling pathway in activated endothelial cells. The fact that these effects are carried out at lower concentrations than those required for other inhibitors of angiogenesis makes AD0157 a new promising drug candidate for further evaluation in the treatment of cancer and other angiogenesis-related pathologies. |
| Databáze: | OpenAIRE |
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