Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy

Autor: Ana Cristina Portugal Pinto de Carvalho, Priscila Bezerra dos Santos Melo, Cândida Hermínia Campos de Magalhães Bertini, Adroaldo Guimarães Rossetti, Celli Rodrigues Muniz
Přispěvatelé: Instituto Agronômico de campinas (IAC), Embrapa Agroindústria Tropical, Universidade Federal do Ceará, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Rok vydání: 2019
Předmět:
Zdroj: REVISTA AGRO@MBIENTE ON-LINE; v. 13 (2019): Edição Continuada; 1-13
Revista Agro@mbiente On-line; v. 13 (2019): Edição Continuada; 1-13
AGRO@MBIENTE
JOURNALRAGR; Vol. 13 (2019): Edição Continuada; 1-13
REVISTA AGRO@MBIENTE ON-LINE; Vol. 13 (2019): Edição Continuada; 1-13
Agro@mbiente on-line
Universidade Federal de Roraima (UFRR)
instacron:UFRR
ISSN: 1982-8470
DOI: 10.18227/1982-8470ragro.v13i0.5333
Popis: Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.
Databáze: OpenAIRE
načítá se...