Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia

Autor: Carla Andrea Alonso, Leila Ben Said, Raoudha Dziri, Karim Ben Slama, Ahlem Jouini, Abdellatif Boudabous, Carmen Torres, Naouel Klibi
Přispěvatelé: Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM), Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire (LR11IPT03), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Universidad de La Rioja (UR), Institut supérieur des sciences biologiques appliquées de Tunis = Higher Institute of Applied Biological Sciences of Tunis (ISSBAT), This work was partially supported by Project SAF2012-35474 from the Ministerio de Economía y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER), and by project from the Tunisian Ministry of Higher Education, Scientific Research and Information and Communication Technologies., We thank A. Caratolli for providing us with positive controls for PBRT testing. Carla Andrea Alonso has a predoctoral fellowship FPI from the Ministerio de Economía y Competitividad of Spain.
Rok vydání: 2014
Předmět:
Klebsiella pneumoniae
medicine.medical_treatment
MESH: Enterobacteriaceae/genetics
MESH: Anti-Bacterial Agents/pharmacology
medicine.disease_cause
Integrons
chemistry.chemical_compound
Soil
Vegetables
MESH: Animals
MESH: Enterobacteriaceae/enzymology
MESH: Water Microbiology
Soil Microbiology
2. Zero hunger
biology
MESH: Vegetables/microbiology
General Medicine
Enterobacteriaceae
Citrobacter freundii
Anti-Bacterial Agents
Bacterial Typing Techniques
Gene cassette
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Integrons/genetics
MacConkey agar
MESH: Tunisia
Water Microbiology
Plasmids
MESH: Soil Microbiology
Tunisia
MESH: Enterobacteriaceae/isolation & purification
Microbiology
MESH: Bacterial Typing Techniques
beta-Lactamases
MESH: Plasmids/genetics
medicine
Food microbiology
Animals
Humans
Escherichia coli
MESH: Humans
E. coli
Water
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
bacterial infections and mycoses
MESH: beta-Lactamases/genetics
chemistry
ESBL
MESH: Enterobacteriaceae/drug effects
Beta-lactamase
bacteria
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Food Science
Zdroj: International Journal of Food Microbiology
International Journal of Food Microbiology, Elsevier, 2015, 203, pp.86-92. ⟨10.1016/j.ijfoodmicro.2015.02.023⟩
ISSN: 1879-3460
0168-1605
Popis: International audience; One-hundred-nine samples of 18 different farms (49 of food-vegetables, 41 of soil and 19 of irrigation water) and 45 vegetable food samples of 13 markets were collected in Tunisia. These samples were inoculated in MacConkey agar plates supplemented with cefotaxime (2 μg/ml). ESBL-producing Enterobacteriaceae (ESBL-Eb) were detected in 10 of the 109 farm samples (vegetables, 8.2%; soil, 7.3%; water, 15.8%), and in 4 of 45 vegetables of markets (8.9%), recovering 15 ESBL-Eb. Isolates and ESBL genes detected were: Escherichia coli (n = 8: 5 blaCTX-M-1, 2 blaCTX-M-15 and one blaCTX-M-14), Citrobacter freundii (n = 4: 3 blaCTX-M-15 and one blaSHV-12), Enterobacter hormaechei (n = 2: 2 blaCTX-M-15) and Klebsiella pneumoniae (n = 1, blaCTX-M-15). The ISEcp1 sequence was found upstream of blaCTX-M genes in 13 of 14 strains (in three cases truncated by IS5), and orf477 or IS903 downstream. Class 1 integrons were detected in five strains and contained two gene cassette arrangements (dfrA17-aadA5 and aadA1). Most isolates tested showed a multiresistant phenotype. All blaCTX-M-15-positive strains carried the aac(6′)-1b-cr gene, that affects to amikacin–tobramycin–kanamycin–ciprofloxacin. Five ESBL-Eb strains carried genes of the qnr family. The 8 ESBL-positive E. coli isolates were typed as: ST58/B1 (n = 3) and ST117/D, ST131/B2, ST10/A, ST23/A, and the new ST3496/D (one strain, each). From 1–2 plasmids were detected in all ESBL-positive E. coli isolates (63–179 kb). The ESBL genes were transferred by conjugation in 4 blaCTX-M-1-positive E. coli strains, and transconjugants acquired a 97 kb IncI1 plasmid. ESBL-Eb isolates are frequently disseminated in vegetable farms and potentially could be transmitted to humans through the food chain.
Databáze: OpenAIRE
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