Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain
Autor: | Satoshi Katahira, Hideki Fukuda, Akihiko Kondo, Atsuko Mizuike |
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Rok vydání: | 2006 |
Předmět: |
Oligosaccharides
Saccharomyces cerevisiae Xylose Ethanol fermentation Lignin Applied Microbiology and Biotechnology Pichia Hydrolysate Fungal Proteins chemistry.chemical_compound Xylose metabolism Aldehyde Reductase Cellulose Pichia stipitis Ethanol biology beta-Glucosidase food and beverages D-Xylulose Reductase General Medicine biology.organism_classification Wood Recombinant Proteins Yeast Kinetics Phosphotransferases (Alcohol Group Acceptor) Aspergillus Biochemistry chemistry Xylulokinase Fermentation Biotechnology |
Zdroj: | Applied Microbiology and Biotechnology. 72:1136-1143 |
ISSN: | 1432-0614 0175-7598 |
Popis: | The sulfuric acid hydrolysate of lignocellulosic biomass, such as wood chips, from the forest industry is an important material for fuel bioethanol production. In this study, we constructed a recombinant yeast strain that can ferment xylose and cellooligosaccharides by integrating genes for the intercellular expressions of xylose reductase and xylitol dehydrogenase from Pichia stipitis, and xylulokinase from Saccharomyces cerevisiae and a gene for displaying beta-glucosidase from Aspergillus acleatus on the cell surface. In the fermentation of the sulfuric acid hydrolysate of wood chips, xylose and cellooligosaccharides were completely fermented after 36 h by the recombinant strain, and then about 30 g/l ethanol was produced from 73 g/l total sugar added at the beginning. In this case, the ethanol yield of this recombinant yeast was much higher than that of the control yeast. These results demonstrate that the fermentation of the lignocellulose hydrolysate is performed efficiently by the recombinant Saccharomyces strain with abilities for xylose assimilation and cellooligosaccharide degradation. |
Databáze: | OpenAIRE |
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