Long-term voluntary running modifies the levels of proteins of the excitatory/inhibitory system and reduces reactive astrogliosis in the brain of Ts65Dn mouse model for Down syndrome
| Autor: | Elizabeth Kida, Giorgio Albertini, Adam A. Golabek, Marius Walus |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Glutamate decarboxylase Mice Transgenic Receptors Metabotropic Glutamate Mice 03 medical and health sciences Glutamatergic 0302 clinical medicine Receptors GABA Physical Conditioning Animal Internal medicine Glutamine synthetase medicine Animals Gliosis Receptor Molecular Biology Glial fibrillary acidic protein biology Glutamate Decarboxylase General Neuroscience Brain medicine.disease Astrogliosis Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology Excitatory Amino Acid Transporter 2 biology.protein Metabotropic glutamate receptor 1 Female Neurology (clinical) Down Syndrome 030217 neurology & neurosurgery Developmental Biology Ionotropic effect |
| Zdroj: | Brain Research. 1766:147535 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2021.147535 |
| Popis: | We showed previously that voluntary long-term running improved cognition and motor skills, but in an age-dependent manner, in the Ts65Dn mouse model for Down syndrome (DS). Presently, we investigated the effect of running on the levels of some key proteins of the excitatory/inhibitory system, which is impaired in the trisomic brain, and on astroglia, a vital component of this system. Ts65Dn mice had free access to a running wheel for 9–13 months either from weaning or from the age of 7 months. Sedentary Ts65Dn mice served as controls. We found that running modified the levels of four of the seven proteins we tested that are associated with the glutamatergic/GABA–ergic system. Thus, Ts65Dn runners demonstrated increased levels of glutamine synthetase and metabotropic glutamate receptor 1 and decreased levels of glutamate transporter 1 and glutamic acid decarboxylase 65 (GAD65) versus sedentary mice, but of metabotropic glutamate receptor 1 and GAD65 only in the post-weaning cohort. GAD67, ionotropic N-methyl-D-aspartate type receptor subunit 1, and GABAAα5 receptors’ levels were similar in runners and sedentary animals. The number of glial fibrillary acidic protein (GFAP)–positive astrocytes and the levels of GFAP were significantly reduced in runners relative to sedentary mice. Our study provides new insight into the mechanisms underlying the beneficial effect of voluntary, sustained running on function of the trisomic brain by identifying the involvement of proteins associated with glutamatergic and GABAergic systems and reduction in reactive astrogliosis. |
| Databáze: | OpenAIRE |
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