Sperm cryostorage in a dry tank: An accurate alternative

Autor: Florence Brugnon, Laurent Janny, Cyril Bouche, Aurélie Vega, Stéphanie Mestres, Bruno Pereira, Laure Chaput, Hanae Pons-Rejraji, Vorilhon S
Přispěvatelé: Imagerie Moléculaire et Stratégies Théranostiques - Clermont Auvergne (IMoST), Université Clermont Auvergne (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), service de Biostatistiques, DRCI, Centre Hospitalier Universitaire de Clermont-Ferrand, Laboratoire BDR CECOS - FIV AMP, CHU Estaing [Clermont-Ferrand], Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Clermont-Ferrand, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), BRUGNON, Florence
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Adult
Male
Acrosome integrity
[SDV]Life Sciences [q-bio]
Human sperm
Liquid phase
Semen
DNA fragmentation
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Semen analysis
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Vitality
General Biochemistry
Genetics and Molecular Biology
Liquid nitrogen tank
03 medical and health sciences
0302 clinical medicine
Animal science
Dry tank
medicine
Humans
[SDV.BDD]Life Sciences [q-bio]/Development Biology
ComputingMilieux_MISCELLANEOUS
Slow freezing
Cryopreservation
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Chemistry
urogenital system
[SDV.BA]Life Sciences [q-bio]/Animal biology
0402 animal and dairy science
Floor level
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Motility
04 agricultural and veterinary sciences
General Medicine
Liquid nitrogen
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
040201 dairy & animal science
Sperm
Spermatozoa
[SDV] Life Sciences [q-bio]
Dry storage
General Agricultural and Biological Sciences
Cryostorage
Semen Preservation
Zdroj: Cryobiology
Cryobiology, Elsevier, 2020, 94, pp.32-39. ⟨10.1016/j.cryobiol.2020.05.002⟩
ISSN: 0011-2240
1090-2392
Popis: International audience; This prospective study aimed to determine the effects of dry nitrogen cryostorage on human sperm characteristics in comparison with liquid nitrogen cryostorage. For this purpose, 42 men undergoing routine semen analysis (21 normozoospermia and 21 with altered semen parameters) were analyzed. After slow freezing, half of the straws of each sample were randomly stored in liquid and dry tanks, at the top and bottom levels of the latter. After 6 months storage, thawed samples were treated by density gradient centrifugation and sperm characteristics were compared. There was no difference in sperm progressive motility (15.1% ± 14.2% vs. 15.1% ± 12.7%; p = 0.76), sperm vitality (25.5% ± 17.7% vs. 26.2% ± 19%; p = 0.71), percentages of acrosome-reacted spermatozoa (38% ± 8.5% vs. 38.5% ± 7.4%; p = 0.53) and DNA fragmentation spermatozoa (27.3% ± 12.4% vs. 28.5% ± 12.9%, p = 0.47) after cryostorage in the dry or the liquid nitrogen tank. Moreover, we did not observe differences between either cryostorage system for normal and altered sperm samples. This lack of difference was also observed whatever the floor level of cryostorage in the dry tank. The temperature measurement of the dry tank showed a stable temperature at -194 °C throughout storage whatever the storage floor level, guaranteeing the stability of the low temperatures suitable for human sperm storage. Because of its greater safety, dry storage without contact with the liquid phase should be preferred and can be a useful alternative for the cryostorage of human sperm samples.
Databáze: OpenAIRE
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