Fish oil supplementation increases expression of mammary tumor apoptosis mediators and reduces inflammation in an obesity-associated HER-2 breast cancer model
| Autor: | Amber L. Hutchinson, Hannah R. Wellings, Krista A. Power, Nadia M Cartwright, Lindsay E. Robinson, David W.L. Ma, William J. Muller, Danyelle M. Liddle, Jennifer M. Monk, Jessie L. Burns |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Receptor ErbB-2 Endocrinology Diabetes and Metabolism Clinical Biochemistry Apoptosis Breast Neoplasms Biochemistry Mice Random Allocation 03 medical and health sciences Fish Oils Mammary Glands Animal 0302 clinical medicine Cell Line Tumor Internal medicine Animals Humans Medicine Obesity RNA Messenger Molecular Biology Inflammation Mammary tumor Nutrition and Dietetics biology business.industry Leptin Body Weight Fatty Acids Menhaden Mammary Neoplasms Experimental Fish oil biology.organism_classification medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology Endocrinology Adipose Tissue 030220 oncology & carcinogenesis Dietary Supplements Female Resistin business Corn oil Hormone |
| Zdroj: | The Journal of Nutritional Biochemistry. 95:108763 |
| ISSN: | 0955-2863 |
| Popis: | Obesity is associated with inflammation and has been shown to increase breast cancer severity. The objective of this study was to examine the effect of fish oil (FO) supplementation in obesity-associated mammary tumorigenesis in the MMTV-neu(ndl)-YD5 mouse model of human epidermal growth factor receptor-2 positive BC. Female mice were fed one of three diets for 16 weeks: i) high fat diet [HF, % kacl: 41.2% lard, 18.7% corn oil (CO)], ii) an isocaloric HF plus menhaden FO diet (HF+FO, % kcal: 41.2 lard, 13.4% CO, 5.3% FO), iii) low fat diet (LF, % kcal: 4.7% lard, 6% CO). HF mice had increased body weight, visceral adipose weight and serum hormone concentrations (increased leptin and resistin; decreased adiponectin) versus LF, which was attenuated in the HF+FO group versus HF (P.05). Compared to HF, tumor onset was delayed in HF+FO and LF mice (P0.05). Compared to HF, HF+FO reduced mammary tumor multiplicity (-27%), tumor weight (-46%) and total tumor volume (-50%) (P0.05). Additionally, HF+FO reduced mammary tumor multiplicity (-33%), tumor weight (-39%) and total tumor volume (-60%) versus LF. HF+FO improved mammary tumor apoptosis status with increased expression of pro-apoptotic Bad and decreased expression of anti-apoptotic Bcl-xLmediators versus HF (P0.05). Additionally, HF+FO decreased tumor protein expression of activated Akt, NFκB p65 and STAT3, versus HF (P0.05). Tumor mRNA expression of inflammatory mediators TNFα, IL-6 and leptin were reduced in HF+FO, whereas IL-10 expression was increased compared to HF (P0.05). Collectively these results demonstrate the efficacy of FO supplementation for improving obesity-associated breast cancer outcomes. |
| Databáze: | OpenAIRE |
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