Anticariogenic Activities ofIDerris reticulata/IEthanolic Stem Extract AgainstIStreptococcus mutans/I
| Autor: | Wanwisa Khunawattanakul, Achida Jaruchotikamol, Sakulrat Rattanakiat, Patcharapa Modtaku, Pawitra Pulbutr, Natnicha Phetsaardeiam, Rattazart Denchai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sucrose 030106 microbiology Microbial Sensitivity Tests Dental Caries medicine.disease_cause Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Anticarcinogenic Agents Pathogen Minimum bactericidal concentration biology Traditional medicine Ethanol Plant Extracts Biofilm Pathogenic bacteria 030206 dentistry biology.organism_classification Antimicrobial Anti-Bacterial Agents Derris chemistry Biofilms Antibacterial activity Agronomy and Crop Science |
| Zdroj: | Pakistan journal of biological sciences : PJBS. 21(6) |
| ISSN: | 1028-8880 |
| Popis: | BACKGROUND AND OBJECTIVE Streptococcus mutans is a dominant causative pathogen of dental caries, which is a major oral health problem affecting million people worldwide. Derris reticulata is a medicinal plant possessing antimicrobial activity against several Gram-positive pathogenic bacteria. None the less, its effects on growth and cariogenic properties of S. mutans has not been clearly established. This study aimed to investigate the antibacterial and anti cariogenic activities of the D. reticulata ethanolic stem extract. MATERIALS AND METHODS The TLC analysis was performed to authenticate the D. reticulata sample. Minimum inhibition concentration and minimum bactericidal concentration were determined by using broth dilution and drop plate methods, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to evaluate the anticariogenic activity. RESULTS The ethanolic stem extract of D. reticulata possessed the antibacterial activity against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL-1, respectively. The extract at the lower concentrations of sub-MIC also had significant inhibitory actions against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production. CONCLUSION The D. reticulata stem extract had a substantial anticariogenic activities and thus potentially be developed as an oral health care product for dental caries prevention in the near future. |
| Databáze: | OpenAIRE |
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