Caged Cumate Enables Proximity-Dependent Control Over Gene Expression
| Autor: | Sabrina H. Tran, Anna C. Love, Jennifer A. Prescher |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cell
Gene Expression caged probe 010402 general chemistry 01 natural sciences Biochemistry Article cellular contact Medicinal and Biomolecular Chemistry Gene expression medicine Genetics Inducer Molecular Biology 010405 organic chemistry Activator (genetics) Chemistry Organic Chemistry imaging cumate Small molecule 0104 chemical sciences Cell biology gene transcription medicine.anatomical_structure Molecular Medicine Generic health relevance Biochemistry and Cell Biology Target gene |
| Zdroj: | Chembiochem : a European journal of chemical biology, vol 22, iss 14 Chembiochem |
| Popis: | Cell-cell interactions underlie diverse physiological processes yet remain challenging to examine with conventional imaging tools. Here we report a novel strategy to illuminate cell proximity using transcriptional activators. We repurposed cumate, a small molecule inducer of gene expression, by caging its key carboxylate group with a nitrile. Nitrilase-expressing activator cells released the cage, liberating cumate for consumption by reporter cells. Reporter cells comprising a cumate-responsive switch expressed a target gene when in close proximity to the activator cells. Overall, this strategy provides a versatile platform to image and potentially manipulate cellular interactions over time. |
| Databáze: | OpenAIRE |
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