Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine

Autor:	Maria Isabel de Oliveira Penteado, M. T. Pozzobon, C.B. do Valle, A. A. Pinheiro, Vera Tavares de Campos Carneiro
Rok vydání:	2019
Předmět:	biology fungi food and beverages Plant Science General Medicine biology.organism_classification Brachiaria chemistry.chemical_compound chemistry Apomixis Shoot Botany Colchicine Kinetin Cultivar Ploidy Agronomy and Crop Science Explant culture
Zdroj:	Plant cell reports. 19(3)
ISSN:	1432-203X
Popis:	Some species of Brachiaria, generally tetraploid apomictic varieties, have become important forage grasses in the tropics. Breeding of Brachiaria depends on compatibility with the available apomitic tretraploid cultivars. This paper describes a procedure for chromosome duplication of two Bracharia brizantha diploid sexual accessions, using colchicine treatment of basal segments of in-vitro-grown plants. Explants were cultured on a medium containing 1 mg/l naphthaleneacetic acid, 3 mg/l kinetin and 0.01% colchicine for 48 h and transferred to the same medium without colchicine until shoot regeneration occurred. Regenerated plants were screened by flow cytometry, and chromosome number duplication was confirmed by cytological analysis of root tips.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0c3dfbc5bd1c775fca45bb86a1b5588 https://pubmed.ncbi.nlm.nih.gov/30754907 Zobrazit plný text záznamu Full text from SpringerLink