Iron accumulation in skeletal muscles of old mice is associated with impaired regeneration after ischaemia–reperfusion damage
| Autor: | Gordon S. Lynch, Francesca M. Alves, Scott Ayton, Ashley I. Bush, Justin P. Hardee, Kai Kysenius, Marissa K. Caldow, René Koopman, Peter J. Crouch |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Sarcopenia Iron Lipid peroxidation Diseases of the musculoskeletal system medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ischemia Physiology (medical) Internal medicine medicine Animals Regeneration Orthopedics and Sports Medicine Muscle Skeletal Wasting business.industry QM1-695 Skeletal muscle Muscle mass Original Articles medicine.disease Muscle atrophy 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry RC925-935 Ageing Reperfusion Injury 030220 oncology & carcinogenesis Human anatomy Original Article medicine.symptom business Reperfusion injury Oxidative stress |
| Zdroj: | Journal of Cachexia, Sarcopenia and Muscle, Vol 12, Iss 2, Pp 476-492 (2021) Journal of Cachexia, Sarcopenia and Muscle |
| ISSN: | 2190-5991 2190-6009 |
| Popis: | Background Oxidative stress is implicated in the insidious loss of muscle mass and strength that occurs with age. However, few studies have investigated the role of iron, which is elevated during ageing, in age‐related muscle wasting and blunted repair after injury. We hypothesized that iron accumulation leads to membrane lipid peroxidation, muscle wasting, increased susceptibility to injury, and impaired muscle regeneration. Methods To examine the role of iron in age‐related muscle atrophy, we compared the skeletal muscles of 3‐month‐old with 22‐ to 24‐month‐old 129SvEv FVBM mice. We assessed iron distribution and total elemental iron using laser ablation inductively coupled plasma mass spectrometry and Perls' stain on skeletal muscle cross‐sections. In addition, old mice underwent ischaemia–reperfusion (IR) injury (90 min ischaemia), and muscle regeneration was assessed 14 days after injury. Immunoblotting was used to determine lipid peroxidation (4HNE) and iron‐related proteins. To determine whether muscle iron content can be altered, old mice were treated with deferiprone (DFP) in the drinking water, and we assessed its effects on muscle regeneration after injury. Results We observed a significant increase in total elemental iron (+43%, P |
| Databáze: | OpenAIRE |
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