Characterization of T-cell surface proteins bound by heterologous antisera to antigen-specific T-cell products
| Autor: | R W Rosenstein, Wlodzimierz Ptak, Robert E. Cone, J H Murray, G M Iverson, Richard K. Gershon |
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| Rok vydání: | 1981 |
| Předmět: |
Antiserum
Multidisciplinary Effector Immune Sera T-Lymphocytes T cell Radioimmunoassay Fluorescent Antibody Technique Membrane Proteins Heterologous Mice Inbred Strains Antigen-Antibody Complex Biology Stain Molecular biology Molecular Weight Mice medicine.anatomical_structure Membrane protein Antigens Surface medicine Animals Receptor Antigen-presenting cell Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 78:6411-6415 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.78.10.6411 |
| Popis: | Heterologous antisera specific for murine T-cell antigen-recognition molecules were prepared by immunization of rabbits with dinitrophenyl-specific murine T-cell suppressor factors that had been purified by hapten-affinity chromatography. The antisera (i) bind to antigen-specific T-cell products that differ in their antigen-recognizing specificity; (ii) absorb the specific suppressor activity in preparations containing suppressor factors; (iii) stain all Lyt2+ T cells brightly in indirect immunofluorescence examination, stain some Lyt1+ cells (with low intensity), and do not stain B cells; (iv) precipitate cell membrane proteins from T cells that bear striking structural resemblance to the antigen-specific molecules used for immunization. These results suggest that, like B cells, there is a commonality between antigen-specific effector molecules released by T cells and their membrane-associated receptors. |
| Databáze: | OpenAIRE |
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