Freeze Fracturing and Freeze Drying of Renal Tissue for Scanning Electron Microscopy
| Autor: | E. E. Wheeler, P. B. Herdson |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Anti-Glomerular Basement Membrane Disease Renal glomerulus Scanning electron microscope Biopsy Kidney Glomerulus Freeze Fracturing Kidney urologic and male genital diseases Freeze-drying Methods medicine Animals Humans Histological Techniques Renal tissue General Medicine Capillaries Rats Freeze Drying Kidney Tubules medicine.anatomical_structure Evaluation Studies as Topic Transmission electron microscopy Microscopy Electron Scanning Biomedical engineering |
| Zdroj: | American Journal of Clinical Pathology. 60:229-233 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/60.2.229 |
| Popis: | The application of freeze-fracturing and freeze-drying technics to scanning electron microscopy of renal tissue is examined. Straightforward, reliable methods for fracturing and handling of such material during freezedying and vacuum coating are described. With these technics, the renal glomerulus either fractures right across or Bowman’s capsule “shells off” to reveal the underlying capillary surfaces. The former situation allows correlation with light and transmission electron microscopy, while the latter is useful for studying the three-dimensional structure of the podocytes. The technics are applicable to the study of human renal biopsies, as exemplified by preliminary comments concerning a case of “Goodpasture’s syndrome.” |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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