Methods to characterize protein interactions with β-arrestin in cellulo
| Autor: | Revu Ann Alexander, Hervé Enslen, Isaure Lot |
|---|---|
| Přispěvatelé: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Scaffold protein Co-immunoprecipitation Yeast two hybrid media_common.quotation_subject Two-hybrid screening [SDV]Life Sciences [q-bio] [SDV.BC]Life Sciences [q-bio]/Cellular Biology Protein–protein interaction Scaffold 03 medical and health sciences 0302 clinical medicine [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Arrestin Internalization Cytoskeleton Receptor Bioluminescence resonance energy transfer (BRET) media_common G protein-coupled receptor GST pull-down Chemistry β-Arrestin Cell biology 030104 developmental biology 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology, beta-arrestins Methods in Molecular Biology, beta-arrestins, 1957, pp.139-158, 2019, ⟨10.1007/978-1-4939-9158-7_9⟩ Beta-Arrestins ISBN: 9781493991570 |
| DOI: | 10.1007/978-1-4939-9158-7_9⟩ |
| Popis: | International audience; β-Arrestins 1 and 2 (β-arr1 and β-arr2) are ubiquitous proteins with common and distinct functions. They were initially identified as proteins recruited to stimulated G protein-coupled receptors (GPCRs), regulating their desensitization and internalization. The discovery that β-arrs could also interact with more than 400 non-GPCR protein partners brought to light their central roles as multifunctional scaffold proteins regulating multiple signalling pathways from the plasma membrane to the nucleus, downstream of GPCRs or independently from these receptors. Through the regulation of the activities and subcellular localization of their binding partners, β-arrs control various cell processes such as proliferation, cytoskeletal rearrangement, cell motility, and apoptosis. Thus, the identification of β-arrs binding partners and the characterization of their mode of interaction in cells are central to the understanding of their function. Here we provide methods to explore the molecular interaction of β-arrs with other proteins in cellulo. |
| Databáze: | OpenAIRE |
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