Balancing cell migration with matrix degradation enhances gene delivery to cells cultured three-dimensionally within hydrogels
| Autor: | Lonnie D. Shea, Jaclyn A. Shepard, Ariella Shikanov, Alyssa Huang |
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| Rok vydání: | 2010 |
| Předmět: |
Population
Cell Culture Techniques Pharmaceutical Science Gene delivery Transfection Article Polyethylene Glycols Extracellular matrix Mice Tissue engineering Cell Movement Cell Adhesion Animals Transgenes education education.field_of_study Tissue Engineering Chemistry Genetic transfer Gene Transfer Techniques technology industry and agriculture Hydrogels Cell migration DNA Molecular biology Matrix Metalloproteinases Extracellular Matrix Cross-Linking Reagents Cell culture Self-healing hydrogels NIH 3T3 Cells Biophysics Oligopeptides Plasmids |
| Zdroj: | Journal of Controlled Release. 146:128-135 |
| ISSN: | 0168-3659 |
| Popis: | In regenerative medicine, hydrogels are employed to fill defects and support the infiltration of cells that can ultimately regenerate tissue. Gene delivery within hydrogels targeting infiltrating cells has the potential to promote tissue formation, but the delivery efficiency of non-viral vectors within hydrogels is low, hindering their applicability in tissue regeneration. To improve their functionality, we have conducted a mechanistic study to investigate the contribution of cell migration and matrix degradation on gene delivery. In this report, lipoplexes were entrapped within hydrogels based on poly(ethylene glycol) (PEG) crosslinked with peptides containing matrix metalloproteinase degradable sequences. The mesh size of these hydrogels is substantially less than the size of the entrapped lipoplexes, which can function to retain vectors. Cell migration and transfection were simultaneously measured within hydrogels with varying density of cell adhesion sites (Arg-Gly-Asp peptides) and solids content. Increasing RGD density increased expression levels up to 100-fold, while greater solids content sustained expression levels for 16 days. Increasing RGD density and decreasing solids content increased cell migration, which indicates expression levels increase with increased cell migration. Initially exposing cells to vector resulted in transient expression that declined after 2 days, verifying the requirement of migration to sustain expression. Transfected cells were predominantly located within the population of migrating cells for hydrogels that supported cell migration. Although the small mesh size retained at least 70% of the lipoplexes in the absence of cells after 32 days, the presence of cells decreased retention to 10% after 16 days. These results indicate that vectors retained within hydrogels contact migrating cells, and that persistent cell migration can maintain elevated expression levels. Thus, matrix degradation and cell migration are fundamental design parameters for maximizing gene delivery within hydrogels. |
| Databáze: | OpenAIRE |
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