Development of a pregnancy-specific reference material for thyroid biomarkers, vitamin D, and nutritional trace elements in serum
| Autor: | Denise S. Tevis, Lisa E. Kilpatrick, Ashley S.P. Boggs, Brittany L. Kassim, Ravinder J. Singh, Jennifer V. Kemp, Michael A. Nelson, Carolyn Q. Burdette, Stefan K.G. Grebe, Stephen E. Long, Zachary A Fultz, Jeffery M. Jarrett, Stephen A. Wise |
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| Rok vydání: | 2020 |
| Předmět: |
Vitamin
medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Thyroid Gland Thyrotropin 030209 endocrinology & metabolism Thyroglobulin Article 03 medical and health sciences chemistry.chemical_compound Selenium 0302 clinical medicine Thyroid-stimulating hormone Pregnancy Tandem Mass Spectrometry Internal medicine medicine Vitamin D and neurology Humans 030212 general & internal medicine Vitamin D Triiodothyronine medicine.diagnostic_test Biochemistry (medical) Thyroid General Medicine Vitamins Reverse triiodothyronine Trace Elements Zinc Endocrinology medicine.anatomical_structure chemistry Immunoassay Female Biomarkers Copper Chromatography Liquid |
| Zdroj: | Clin Chem Lab Med |
| ISSN: | 1437-4331 |
| Popis: | Objectives Matrix differences among serum samples from non-pregnant and pregnant patients could bias measurements. Standard Reference Material 1949, Frozen Human Prenatal Serum, was developed to provide a quality assurance material for the measurement of hormones and nutritional elements throughout pregnancy. Methods Serum from non-pregnant women and women in each trimester were bottled into four levels based on pregnancy status and trimester. Liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were developed and applied to the measurement of thyroid hormones, vitamin D metabolites, and vitamin D-binding protein (VDBP). Copper, selenium, and zinc measurements were conducted by inductively coupled plasma dynamic reaction cell MS. Thyroid stimulating hormone (TSH), thyroglobulin (Tg), and thyroglobulin antibody concentrations were analyzed using immunoassays and LC-MS/MS (Tg only). Results Certified values for thyroxine and triiodothyronine, reference values for vitamin D metabolites, VDBP, selenium, copper, and zinc, and information values for reverse triiodothyronine, TSH, Tg, and Tg antibodies were assigned. Significant differences in serum concentrations were evident for all analytes across the four levels (p≤0.003). TSH measurements were significantly different (p Conclusions The analyte concentrations’ changes consistent with the literature and the demonstration of matrix interferences in immunoassay Tg measurements indicate the functionality of this material by providing a relevant matrix-matched reference material for the different stages of pregnancy. |
| Databáze: | OpenAIRE |
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