Differential expression proteomics of human colorectal cancer based on a syngeneic cellular model for the progression of adenoma to carcinoma
| Autor: | Anthony P. Corfield, Stefan Müller, Udo Roth, Katja Engelmann, Stephan Baldus, Tilo Schwientek, Julia Hommer, Franz-Georg Hanisch, Christos Paraskeva, Hanieh Razawi, Georgios Patsos |
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| Rok vydání: | 2009 |
| Předmět: |
Adenoma
Proteomics medicine.medical_specialty Proteome Clinical Biochemistry Down-Regulation Steroid biosynthesis Biology Models Biological Biochemistry Peptide mass fingerprinting Western blot Cell Line Tumor Internal medicine medicine Protein biosynthesis Humans Electrophoresis Gel Two-Dimensional Phosphoserine Aminotransferase education Molecular Biology education.field_of_study medicine.diagnostic_test Prostaglandin E synthase 2 Molecular biology Up-Regulation Endocrinology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Disease Progression biology.protein Colorectal Neoplasms Villin |
| Zdroj: | PROTEOMICS. 10:194-202 |
| ISSN: | 1615-9853 |
| DOI: | 10.1002/pmic.200900614 |
| Popis: | This is the first differential expression proteomics study on a human syngeneic cellular in vitro progression model of the colorectal adenoma-to-carcinoma sequence, the anchorage-dependent non-tumorigenic adenoma derived cell line AA/C1 and the derived anchorage-independent and tumorigenic carcinoma cell line AA/C1/SB10C. The study is based on quantitative 2-DE and is complemented by Western blot validation. Excluding redundancies due to proteolysis and post-translational modified isoforms of over 2000 protein spots, 13 proteins were revealed as regulated with statistical variance being within the 95th confidence level and were identified by peptide mass fingerprinting in MALDI MS. Progression-associated proteins belong to the functional complexes of anaerobic glycolysis/gluconeogenesis, steroid biosynthesis, prostaglandin biosynthesis, the regulation and maintenance of the cytoskeleton, protein biosynthesis and degradation, the regulation of apoptosis or other functions. Partial but significant overlap was revealed with previous proteomics and transcriptomics studies in colorectal carcinoma. Among upregulated proteins we identified 3-HMG-CoA synthase, protein phosphatase 1, prostaglandin E synthase 2, villin 1, annexin A1, triosephosphate isomerase, phosphoserine aminotransferase 1, fumarylacetoacetate hydrolase and pyrroline-5-carboxylate reductase 1 (PYCR1), while glucose-regulated protein 78, cathepsin D, lamin A/C and quinolate phosphoribosyltransferase were downregulated. |
| Databáze: | OpenAIRE |
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