Effect of glutathione levels on aflatoxin B1-DNA binding in livers and kidneys of male rats and hamsters pretreated with buthionine sulfoximine and diethylmaleate
| Autor: | Prathima Gopalan-Kriczky, Prabhakar D. Lotlikar, Shingo Hiruma |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Antimetabolites Antineoplastic Cancer Research medicine.medical_specialty Aflatoxin B1 Time Factors medicine.medical_treatment Intraperitoneal injection Hamster Kidney DNA Adducts chemistry.chemical_compound Cricetinae Methionine Sulfoximine Internal medicine medicine Animals Buthionine sulfoximine Buthionine Sulfoximine Biotransformation Carcinogen Glutathione Transferase Mesocricetus biology Maleates Kidney metabolism DNA Glutathione biology.organism_classification Rats Inbred F344 Rats Endocrinology Liver Oncology Biochemistry chemistry Toxicity |
| Zdroj: | Cancer Letters. 76:25-30 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(94)90130-9 |
| Popis: | The effects of pretreatment of buthionine sulfoximine (BSO) alone or in combination with diethylmaleate (DEM) on glutathione (GSH) levels and aflatoxin B1 (AFB1)-DNA binding have been examined in livers and kidneys of young male Fischer rats and Syrian golden hamsters 2 h after an intraperitoneal injection of [3H]AFB1 (0.4 mg/kg body wt.). Animals were treated with BSO (4 mmol/kg body wt.) alone at 4 h and 2 h or with DEM (3 mmol/kg body wt.) at 4 h and BSO at 2 h before AFB1 injection. Hepatic AFB1-DNA binding was about 29.0 and 6.0 pmol/mg DNA in rats and hamsters, respectively. In rats, BSO increased AFB1-DNA binding by about 40% with a drop in GSH by 70%. Treatment with DEM-BSO increased AFB1-DNA binding by about 80% with a concomitant drop in GSH in both species. In hamsters, BSO increased AFB1-DNA binding by only 10% with a 50% drop in GSH. The kidneys of both species have lower GSH levels and AFB1-DNA binding than their respective liver tissues. The effect of BSO alone or in combination with DEM on both GSH levels and AFB1-DNA binding are comparable even though BSO alone is less effective in both species. The role of modulation of GSH levels on AFB1-DNA binding and hence biological effects of AFB1 in these two species is discussed. |
| Databáze: | OpenAIRE |
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