Synthesis and Use of Fluorescent Molecular Probes for Measuring Cell-Surface Enzymatic Oxidation of Amino Acids and Amines in Seawater
| Autor: | Brian Palenik, James F. Marecek, Cindy Lee, Silvio Pantoja |
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| Rok vydání: | 1993 |
| Předmět: |
Photochemistry
Lysine Biophysics Deamination L-Amino Acid Oxidase Biochemistry chemistry.chemical_compound Cadaverine Organic chemistry Seawater Amines Amino Acids Molecular Biology Fluorescent Dyes chemistry.chemical_classification Lucifer yellow Bacteria Hydrolysis Cell Membrane Eukaryota Membrane Proteins Oxidative deamination Hydrogen Peroxide Cell Biology Isoquinolines Fluorescence Combinatorial chemistry Amino acid Amino Acids Neutral chemistry Phytoplankton Amine gas treating Adsorption Amino Acid Oxidoreductases Oxidation-Reduction |
| Zdroj: | Analytical Biochemistry. 211:210-218 |
| ISSN: | 0003-2697 |
| Popis: | A method for investigating cell-surface enzymatic oxidative deamination of amino acids and amines in sea water was developed. This technique used synthetic fluorescent Lucifer Yellow derivatives of the amino acid lysine and the amine cadaverine as molecular probes to investigate oxidation pathways and rates. The probes were chemically stable under the conditions used and did not adsorb to container surfaces. The oxidative deamination of the fluorescent probes added to phyto-plankton cultures and the subsequent production of their fluorescent oxidation products could be selectively detected by HPLC at 250 pM levels. This approach allows selective investigation of cell-surface enzymatic oxidation since neither transport of the probes across the cell membrane nor chemical transformation of the probes occurs. Bacteria were also capable of oxidizing the fluorescent amino acid probe. |
| Databáze: | OpenAIRE |
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