A rabbit monoclonal antibody-mediated lateral flow immunoassay for rapid detection of CTX-M extended-spectrum β-lactamase-producing Enterobacterales
| Autor: | Chiaki Sakuma, Taiji Furukawa, Takashi Shibata, Shinobu Ishigaki, Yasunori Kurosawa, Miwa Asahara, Nami Hatayama, Yasuo Ono, C.J. Okumura, Masataka Nakagawa, Yoshinori Sato, Satoshi Nishida, Hisayo Shimizu, Toshiaki Maruyama, Teruo Akuta, Yuki Ouchi, Yu Nakajima |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
02 engineering and technology Monoclonal antibody Biochemistry Sensitivity and Specificity beta-Lactamases Microbiology law.invention Agar plate 03 medical and health sciences Plasmid Antibiotic resistance Enterobacteriaceae Structural Biology law polycyclic compounds medicine Animals Molecular Biology 030304 developmental biology Detection limit Immunoassay 0303 health sciences biology Chemistry Antibodies Monoclonal General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses 021001 nanoscience & nanotechnology biology.organism_classification Point-of-Care Testing biology.protein Recombinant DNA Rabbits Antibody 0210 nano-technology Bacteria |
| Zdroj: | International journal of biological macromolecules. 185 |
| ISSN: | 1879-0003 |
| Popis: | Infections of CTX-M extended-spectrum β-lactamase-producing Enterobacterales are a severe threat in clinical settings. CTX-M genes on plasmids have been transferred to many Enterobacterales species, and these species have spread, leading to the global problem of antimicrobial resistance. Here, we developed a lateral flow immunoassay (LFIA) based on an anti-CTX-M rabbit monoclonal antibody. This antibody detected CTX-M variants from the CTX-M-9, CTX-M-2, and CTX-M-1 groups expressed in clinical isolates. The LFIA showed 100% sensitivity and specificity with clinical isolates on agar plates, and its limit of detection was 0.8 ng/mL recombinant CTX-M-14. The rabbit monoclonal antibody did not cross-react with bacteria producing other class A β-lactamases, including SHV. In conclusion, we developed a highly sensitive and specific LFIA capable of detecting CTX-M enzyme production in Enterobacterales. We anticipate that our LFIA will become a point-of-care test enabling rapid detection of CTX-M in hospital and community settings as well as a rapid environmental test. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect