μ-Opioid and 5-HT1A receptors in the dorsomedial hypothalamus interact for the regulation of panic-related defensive responses
| Autor: | Hélio Zangrossi, Alana Tercino Frias, Frederico Guilherme Graeff, Camila Marroni Roncon, Paula Shimene de Melo Yamashita, Elisabeth Aparecida Audi, Norberto Cysne Coimbra |
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| Rok vydání: | 2017 |
| Předmět: |
Agonist
Male Serotonin medicine.drug_class Pyridines Hypothalamus Receptors Opioid mu (+)-Naloxone Pharmacology Serotonergic Periaqueductal gray Piperazines 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Opioid receptor Escape Reaction medicine SOMATOSTATINA Animals Periaqueductal Gray Pharmacology (medical) Rats Wistar Endogenous opioid Opioidergic 8-Hydroxy-2-(di-n-propylamino)tetralin Chemistry Naloxone Enkephalin Ala(2)-MePhe(4)-Gly(5) Panic 030227 psychiatry Rats Analgesics Opioid Psychiatry and Mental health DAMGO Receptor Serotonin 5-HT1A Panic Disorder Somatostatin 030217 neurology & neurosurgery |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The dorsomedial hypothalamus (DMH) and the dorsal periaqueductal gray (DPAG) have been implicated in the genesis and regulation of panic-related defensive behaviors, such as escape. Previous results point to an interaction between serotonergic and opioidergic systems within the DPAG to inhibit escape, involving µ-opioid and 5-HT1A receptors (5-HT1AR). In the present study we explore this interaction in the DMH, using escape elicited by electrical stimulation of this area as a panic attack index. The obtained results show that intra-DMH administration of the non-selective opioid receptor antagonist naloxone (0.5 nmol) prevented the panicolytic-like effect of a local injection of serotonin (20 nmol). Pretreatment with the selective μ-opioid receptor (MOR) antagonist CTOP (1 nmol) blocked the panicolytic-like effect of the 5-HT1AR agonist 8-OHDPAT (8 nmol). Intra-DMH injection of the selective MOR agonist DAMGO (0.3 nmol) also inhibited escape behavior, and a previous injection of the 5-HT1AR antagonist WAY-100635 (0.37 nmol) counteracted this panicolytic-like effect. These results offer the first evidence that serotonergic and opioidergic systems work together within the DMH to inhibit panic-like behavior through an interaction between µ-opioid and 5-HT1A receptors, as previously described in the DPAG. |
| Databáze: | OpenAIRE |
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