Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs
| Autor: | Qiana L. Matthews, Tanja D. de Gruijl, Anita G.M. Stam, Rieneke van de Ven, Igor P. Dmitriev, Tracy Jane T.H.D. Eisden, Erik Hooijberg, David T. Curiel, Mert Icyuz, Dafni Chondronasiou |
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| Přispěvatelé: | Medical oncology laboratory, Pathology, CCA - Cancer biology and immunology, AII - Cancer immunology |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Naive T cell T cell Immunology lcsh:Medicine Biology Article Viral vector 03 medical and health sciences dendritic cell targeting 0302 clinical medicine Immune system sentinel lymph node Antigen In vivo Drug Discovery medicine melanoma Pharmacology (medical) Pharmacology Effector Melanoma lcsh:R medicine.disease 030104 developmental biology Infectious Diseases medicine.anatomical_structure 030220 oncology & carcinogenesis MART-1 Cancer research melanoma-specific T cells adenovirus (Ad)5/3 |
| Zdroj: | Vaccines Volume 6 Issue 3 Vaccines, 6(3). Multidisciplinary Digital Publishing Institute (MDPI) Vaccines, Vol 6, Iss 3, p 42 (2018) Chondronasiou, D, Eisden, T-J T H D, Stam, A G M, Matthews, Q L, Icyuz, M, Hooijberg, E, Dmitriev, I, Curiel, D T, de Gruijl, T D & van de Ven, R 2018, ' Improved Induction of Anti-Melanoma T Cells by Adenovirus-5/3 Fiber Modification to Target Human DCs ', Vaccines, vol. 6, no. 3 . https://doi.org/10.3390/vaccines6030042 |
| ISSN: | 2076-393X |
| DOI: | 10.3390/vaccines6030042 |
| Popis: | To mount a strong anti-tumor immune response, non T cell inflamed (cold) tumors may require combination treatment encompassing vaccine strategies preceding checkpoint inhibition. In vivo targeted delivery of tumor-associated antigens (TAA) to dendritic cells (DCs), relying on the natural functions of primary DCs in situ, represents an attractive vaccination strategy. In this study we made use of a full-length MART-1 expressing C/B-chimeric adenoviral vector, consisting of the Ad5 capsid and the Ad3 knob (Ad5/3), which we previously showed to selectively transduce DCs in human skin and lymph nodes. Our data demonstrate that chimeric Ad5/3 vectors encoding TAA, and able to target human DCs in situ, can be used to efficiently induce expansion of functional tumor-specific CD8+ effector T cells, either from a naï ve T cell pool or from previously primed T cells residing in the melanoma-draining sentinel lymph nodes (SLN). These data support the use of Ad3-knob containing viruses as vaccine vehicles for in vivo delivery. &ldquo Off-the-shelf&rdquo DC-targeted Ad vaccines encoding TAA could clearly benefit future immunotherapeutic approaches. |
| Databáze: | OpenAIRE |
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