MCR Approach Revealing Protein, Water, and Lipid Depth Profile in Atopic Dermatitis Patients' Stratum Corneum via in Vivo Confocal Raman Spectroscopy
| Autor: | Tom Cambron, Yueqing Niu, Hongyan Zheng, Karl Shiqing Wei, Lesheng Zhang, Ning Su, Paula J. Ray, Zigang Xu |
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| Rok vydání: | 2019 |
| Předmět: |
Adolescent
Lesion skin Confocal 010402 general chemistry Spectrum Analysis Raman 01 natural sciences Analytical Chemistry Dermatitis Atopic symbols.namesake In vivo medicine Stratum corneum Humans Child Skin Multivariate curve resolution integumentary system Chemistry 010401 analytical chemistry Proteins Water Atopic dermatitis medicine.disease Lipids 0104 chemical sciences medicine.anatomical_structure Child Preschool Biophysics symbols Raman spectroscopy Confocal raman spectroscopy |
| Zdroj: | Analytical chemistry. 91(4) |
| ISSN: | 1520-6882 |
| Popis: | Confocal Raman has been widely used for measuring the water concentration profile inside skin to calculate clinical end points, such as stratum corneum thickness. In this article, multivariate curve resolution was applied to resolve the pure components contained in high frequency (2500-4000 cm-1) in vivo confocal Raman data. Three components were identified by comparing with reference spectra of materials in skin. These three components are water, protein, and lipid. The score values associated with these three components were transformed to mass ratio by leveraging the response factors for protein and lipid in a calibration model utilizing the pure material spectra. The concentration profiles for protein and lipid as a function of depth across the stratum corneum are utilized as new clinical end points. Results from an in vivo study with individuals who experience atopic dermatitis symptoms successfully demonstrated a statistical difference between Raman spectra from nonlesion and lesion skin sites. Trends in the depth profiles of the skin components are consistent with previous literature reports. |
| Databáze: | OpenAIRE |
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