Effect of voluntary running on expression of myokines in brains of rats with depression
| Autor: | Yasser M Elbastwisy, Ahmed M Elnaggar, Maha K. Desouky, Sami A. Algaidi, Shaima M. Almasry, Magda A. Eldomiaty |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Cerebellum Immunology myokines Running 03 medical and health sciences 0302 clinical medicine immune-expression in brain areas Physical Conditioning Animal Myokine medicine Immunology and Allergy Animals Original Research Article Rats Wistar Prefrontal cortex Macrophage Migration-Inhibitory Factors Depression (differential diagnoses) Pharmacology Depression Interleukin-6 Dentate gyrus Brain-Derived Neurotrophic Factor Brain voluntary running Rats 030104 developmental biology medicine.anatomical_structure nervous system Case-Control Studies Histopathology Psychology Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Immunopathology and Pharmacology |
| ISSN: | 2058-7384 0394-6320 |
| Popis: | This study aimed to demonstrate the histopathology and immunoexpression of exercise-derived myokines in dentate gyrus (DG), medial prefrontal cortex (mPFC) and cerebellum of depressed Wistar rats during depression and after practising voluntary running. Depression was developed by forced swimming for 2 weeks. Voluntary running was performed by voluntary running for 3 weeks. Brain sections were processed and immunostained to detect brain-derived neurotrophic factor (BDNF), macrophage migration inhibitory factor (MIF), vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6). ImageJ software was used to measure the optical density (OD). BDNF was expressed in neurons in DG, mPFC and granular and Purkinje cells in cerebellum. MIF was expressed in neurons of sub-granular zone in DG, mPFC and Purkinje cells. VEGF was expressed in many neurons in DG, mPFC and Purkinje cells. IL-6 was expressed in some neurons in DG, in neuropil of mPFC and in Purkinje cells. In depression, the OD of studied myokines significantly decreased in all examined areas. After voluntary running, the OD of myokines significantly increased in all areas. This study defines the immunohistochemical expression of myokines in brain areas in depression and after voluntary running and reveals the involvement of the mPFC and cerebellum in the pathophysiology of depression. |
| Databáze: | OpenAIRE |
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