Exploring Leptospiral proteomes to identify potential candidates for vaccine design against Leptospirosis using an immunoinformatics approach
| Autor: | Vibhisha Vaghasia, Kumari Snehkant Lata, Priyanka Sharma, Jayashankar Das, Swapnil Kumar, Subhash Soni, Shivarudrappa B. Bhairappanvar |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
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Molecular Proteomics 0301 basic medicine Serotype Proteome Protein Conformation Science Epitopes T-Lymphocyte Cross Reactions Major histocompatibility complex Article Epitope Structure-Activity Relationship 03 medical and health sciences 0302 clinical medicine Immune system Bacterial Proteins Antigen medicine Humans Leptospirosis Amino Acid Sequence Leptospira Antigens Bacterial Multidisciplinary biology Computational Biology medicine.disease Virology CTL 030104 developmental biology Bacterial Vaccines Vaccines Subunit biology.protein Peptide vaccine Epitopes B-Lymphocyte Medicine 030215 immunology |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Leptospirosis is the most widespread zoonotic disease, estimated to cause severe infection in more than one million people each year, particularly in developing countries of tropical areas. Several factors such as variable and nonspecific clinical manifestation, existence of large number of serovars and asymptomatic hosts spreading infection, poor sanitation and lack of an effective vaccine make prophylaxis difficult. Consequently, there is an urgent need to develop an effective vaccine to halt its spread all over the world. In this study, an immunoinformatics approach was employed to identify the most vital and effective immunogenic protein from the proteome of Leptospira interrogans serovar Copenhageni strain L1-130 that may be suitable to stimulate a significant immune response aiding in the development of peptide vaccine against leptospirosis. Both B-cell and T-cell (Helper T-lymphocyte (HTL) and cytotoxic T lymphocyte (CTL)) epitopes were predicted for the conserved and most immunogenic outer membrane lipoprotein. Further, the binding interaction of CTL epitopes with Major Histocompatibility Complex class I (MHC-I) was evaluated using docking techniques. A Molecular Dynamics Simulation study was also performed to evaluate the stability of the resulting epitope-MHC-I complexes. Overall, this study provides novel vaccine candidates and may prompt further development of vaccines against leptospirosis. |
| Databáze: | OpenAIRE |
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