Noise induced hearing loss and vestibular dysfunction in the guinea pig
| Autor: | Aldo Ferraresi, Anna Rita Fetoni, Pasqualina Maria Picciotti, Eleonora Gaetani, Diana Troiani, Gaetano Paludetti |
|---|---|
| Přispěvatelé: | Fetoni, A, Ferraresi, A, Picciotti, P, Gaetani, E, Paludetti, G, Troiani, D |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Vascular Endothelial Growth Factor A
Crista ampullaris Linguistics and Language medicine.medical_specialty Time Factors Hearing loss Settore BIO/09 - FISIOLOGIA Blotting Western Guinea Pigs Audiology Language and Linguistics Speech and Hearing Evoked Potentials Auditory Brain Stem otorhinolaryngologic diseases medicine Animals Organ of Corti Cochlea 4-hydroxy-2-noneal Vestibular system Aldehydes business.industry Macula Guinea pig Vascular endothelial growth factor Reflex Vestibulo-Ocular medicine.disease Immunohistochemistry medicine.anatomical_structure Hearing Loss Noise-Induced Vestibule ENDOTHELIAL GROWTH-FACTOR Reflex Vestibule Labyrinth sense organs medicine.symptom Vestibulo–ocular reflex business Noise-induced hearing loss |
| Popis: | This study analysed the acoustic and vestibular functional and morphological modifications in guinea pigs after acoustic trauma. Animals were exposed to noise (6 kHz, at 120 dB SPL for 60 minutes) and then auditory brainstem responses (ABR) and vestibuloocular reflex (VOR) were measured at 6 hours, 1 day, 3, 7, and 21 days after noise. Western blotting and immunostaining for 4-hydroxy-2-noneal (4-HNE) and vascular endothelial growth factor (VEGF) were performed in the cochlear and vestibular regions at 1 and 7 days after noise exposure. A significant decrease of VOR gain was observed on day 1 and the recovery was completed at day 21. ABR threshold values reached a level of 80 dB at day 1 after trauma reaching a value of about 50 dB SPL on day 21. 4-HNE expression, a marker of lipid peroxidation was strongly increased in the cochlea. In the vestibule, 4-HNE immunoreactivity was faint. However, VEGF was up-regulated both in the cochlea and vestibule. In conclusion, the expression of VEGF in both cochlear and vestibular structures suggests a reparative role with potentially therapeutic implications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|