Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota
| Autor: | Alexander Keller, Rosalia Deeken, Ute Hentschel, Hanna Faist |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Agrobacterium vitis Agrobacterium Growing season Virulence digestive system DNA Ribosomal Applied Microbiology and Biotechnology 03 medical and health sciences fluids and secretions Plant Microbiology stomatognathic system Plant Tumors RNA Ribosomal 16S Botany Endophytes Cluster Analysis Gall Vitis Pathogen Phylogeny Soil Microbiology Plant Diseases 2. Zero hunger Bacteria Ecology biology Outbreak Sequence Analysis DNA 15. Life on land biology.organism_classification Biota stomatognathic diseases 030104 developmental biology Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 82:5542-5552 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01131-16 |
| Popis: | Crown gall disease of grapevine is caused by virulent Agrobacterium strains and establishes a suitable habitat for agrobacteria and, potentially, other bacteria. The microbial community associated with grapevine plants has not been investigated with respect to this disease, which frequently results in monetary losses. This study compares the endophytic microbiota of organs from grapevine plants with or without crown gall disease and the surrounding vineyard soil over the growing seasons of 1 year. Amplicon-based community profiling revealed that the dominating factor causing differences between the grapevine microbiota is the sample site, not the crown gall disease. The soil showed the highest microbial diversity, which decreased with the distance from the soil over the root and the graft union of the trunk to the cane. Only the graft union microbiota was significantly affected by crown gall disease. The bacterial community of graft unions without a crown gall hosted transient microbiota, with the three most abundant bacterial species changing from season to season. In contrast, graft unions with a crown gall had a higher species richness, which in every season was dominated by the same three bacteria ( Pseudomonas sp., Enterobacteriaceae sp., and Agrobacterium vitis ). For in vitro -cultivated grapevine plantlets, A. vitis infection alone was sufficient to cause crown gall disease. Our data show that microbiota in crown galls is more stable over time than microbiota in healthy graft unions and that the microbial community is not essential for crown gall disease outbreak. IMPORTANCE The characterization of bacterial populations in animal and human diseases using high-throughput deep-sequencing technologies, such as 16S amplicon sequencing, will ideally result in the identification of disease-specific microbiota. We analyzed the microbiota of the crown gall disease of grapevine, which is caused by infection with the bacterial pathogen Agrobacterium vitis. All other Agrobacterium species were found to be avirulent, even though they lived together with A. vitis in the same crown gall tumor. As has been reported for human cancer, the crown gall tumor also hosted opportunistic bacteria that are adapted to the tumor microenvironment. Characterization of the microbiota in various diseases using amplicon sequencing may help in early diagnosis, to serve as a preventative measure of disease in the future. |
| Databáze: | OpenAIRE |
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