Linkage Mapping of 1454 New Maize Candidate Gene Loci
| Autor: | Alain Charcosset, Laurent Decousset, Delphine Samson, Johann Joets, Nicolas Ribière, Xavier Raffoux, Jean-Pierre Martinant, Anne-Marie Jacob, Alain Murigneux, Matthieu Falque, Céline Ridel, Delphine Dervins |
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| Přispěvatelé: | Génétique Végétale (GV), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Genetics and Genomics in Cereals, BIOGEMMA, Laboratoire d'Etudes de la Réparation de l'ADN (LERA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Verneuil Holding, Limagrain, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biogemma |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Genetic Markers
Candidate gene DNA Complementary Genetic Linkage Centromere Population Investigations Biology Genes Plant Polymerase Chain Reaction Polymorphism Single Nucleotide Zea mays Chromosomes Plant Centimorgan Genetic linkage Complementary DNA Genetics education Crosses Genetic Expressed Sequence Tags Recombination Genetic Expressed sequence tag education.field_of_study Chromosome Mapping Computational Biology Meiosis [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding Genetics Population Hybridization Genetic Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Genetics Genetics, 2005, 170 (4), pp.1957-1966. ⟨10.1534/genetics.104.040204⟩ |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.104.040204⟩ |
| Popis: | Bioinformatic analyses of maize EST sequences have highlighted large numbers of candidate genes putatively involved in agriculturally important traits. To contribute to ongoing efforts toward mapping of these genes, we used two populations of intermated recombinant inbred lines (IRILs), which allow a higher map resolution than nonintermated RILs. The first panel (IBM), derived from B73 × Mo17, is publicly available from the Maize Genetics Cooperation Stock Center. The second panel (LHRF) was developed from F2 × F252 to map loci monomorphic on IBM. We built framework maps of 237 loci from the IBM panel and 271 loci from the LHRF panel. Both maps were used to place 1454 loci (1056 on map IBM_Gnp2004 and 398 on map LHRF_Gnp2004) that corresponded to 954 cDNA probes previously unmapped. RFLP was mostly used, but PCR-based methods were also performed for some cDNAs to map SNPs. Unlike in usual IRIL-based maps published so far, corrected meiotic centimorgan distances were calculated, taking into account the number of intermating generations undergone by the IRILs. The corrected sizes of our framework maps were 1825 cM for IBM_Gnp2004 and 1862 cM for LHRF_Gnp2004. All loci mapped on LHRF_Gnp2004 were also projected on a consensus map IBMconsensus_Gnp2004. cDNA loci formed clusters near the centromeres except for chromosomes 1 and 8. |
| Databáze: | OpenAIRE |
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