Synthesis and Characterization of a Walnut Peptides-Zinc Complex and Its Antiproliferative Activity against Human Breast Carcinoma Cells through the Induction of Apoptosis
| Autor: | Lai Ting, Wenzhen Liao, Luying Chen, Jiaoyan Ren, Zhiqiang Yu, Junning Fu, Sreeprasad T. Sreenivasan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Cell chemistry.chemical_element Apoptosis Breast Neoplasms Juglans Zinc Bioinformatics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biosynthesis Cell Line Tumor medicine Humans Gene Cell Proliferation Cell growth General Chemistry Growth Inhibitors 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Covalent bond 030220 oncology & carcinogenesis Female General Agricultural and Biological Sciences Peptides |
| Zdroj: | Journal of agricultural and food chemistry. 64(7) |
| ISSN: | 1520-5118 |
| Popis: | The walnut peptides and zinc ions were combined to generate a walnut peptides-zinc complex (WP1-Zn) with enhanced antiproliferative ability as well as reduced toxicity. The result indicated that Zn ions were successfully combined with WP1 through Zn-N and Zn-O covalent bonds. WP1-Zn compounds exhibited strong antiproliferative ability against the selected human cell lines, especially MCF-7 cells, whose survival rate reduced to 20.02% after exposure to 300 μg/mL of WP1-Zn for 48 h. WP1-Zn inhibited MCF-7 cell proliferation through inducing cell apoptosis and cell cycle arrest. The results indicated that WP1-Zn induced MCF-7 cell apoptosis via the ROS triggered mitochondrial-mediated pathway and cell surface receptor-mediated pathway. Our work is the first attempt to elucidate the synergic effect of novel walnut peptides and Zn and with the hope of better understanding the antiproliferative action of bioactive peptides and a zinc complex and support the potential application of WP1-Zn as a functional food ingredient or complementary medicine. |
| Databáze: | OpenAIRE |
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