ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome biogenesis
| Autor: | Régis Dieckmann, Jean-Charles Sanchez, Laurent Bezin, Jean-Jacques Diaz, Karine Kindbeiter, Laurent Duret, Yohann Couté, Stephane Belin |
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| Přispěvatelé: | Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Biomedical Proteomics Research Group (BPRG), Centre médical universitaire, Idéalp' Pharma, Idealp, Bioinformatique, phylogénie et génomique évolutive (BPGE), Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Physiologie intégrative, cellulaire et moléculaire (PICM), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Exosome complex
Nucleolus Exodeoxyribonucleases/chemistry/metabolism Ribosome biogenesis Biochemistry Analytical Chemistry Substrate Specificity Mutant Proteins/metabolism 0302 clinical medicine Exoribonuclease Large ribosomal subunit RNA Precursors RNA Precursors/metabolism Phylogeny Genetics 0303 health sciences Cell biology Protein Transport 030220 oncology & carcinogenesis Vertebrates Saccharomyces cerevisiae Proteins/genetics Ribosomal Proteins/metabolism Recombinant Fusion Proteins/metabolism Exoribonuclease activity Cell Nucleolus Protein Binding Ribosomal Proteins [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Saccharomyces cerevisiae Proteins Recombinant Fusion Proteins Molecular Sequence Data Biology 03 medical and health sciences Animals Humans Immunoprecipitation Amino Acid Sequence Gene Silencing Ribosomes/metabolism ddc:576 Molecular Biology 030304 developmental biology Computational Biology RNA Ribosomal/metabolism Cell Nucleolus/enzymology Ribosomal RNA Protein Structure Tertiary Protein Structure Tertiary Exodeoxyribonucleases RNA Ribosomal Hela Cells Mutant Proteins Ribosomes Biogenesis HeLa Cells |
| Zdroj: | Molecular and Cellular Proteomics, Vol. 7, No 3 (2008) pp. 546-559 Molecular and Cellular Proteomics Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2007, epub ahead of print. ⟨10.1074/mcp.M700510-MCP200⟩ Molecular and Cellular Proteomics, 2008, 7, epub ahead of print. ⟨10.1074/mcp.M700510-MCP200⟩ Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2008, 7, pp.546-559 |
| ISSN: | 1535-9476 1535-9484 |
| DOI: | 10.1074/mcp.M700510-MCP200⟩ |
| Popis: | International audience; Proteomic analyses of human nucleoli provided molecular bases for an understanding of the multiple functions fulfilled by these nuclear domains. However, the biological roles of about 100 of the identified proteins are unpredictable. The present article describes the functional characterization of one of these proteins, ISG20L2. We demonstrate that ISG20L2 is a 3' to 5' exoribonuclease involved in ribosome biogenesis at the level of 5.8S rRNA maturation, more specifically in the processing of the 12S pre-rRNA. The use of truncated forms of ISG20L2 demonstrated that its N-terminal half promotes the nucleolar localization and suggested that its C-terminal half bears the exoribonuclease activity. Identification of the binding partners of ISG20L2 confirms its involvement in the biogenesis of the large ribosomal subunit. These results strongly support the notion that, in human, as it was demonstrated in yeast, 5.8S rRNA maturation requires several proteins in addition to the exosome complex. Furthermore, this observation sustains greatly the idea that the extremely conserved need for correctly processed rRNAs in vertebrates and yeast are achieved by close but different mechanisms. |
| Databáze: | OpenAIRE |
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