Enhancement of cytokine‐driven NK cell IFN‐γ production after vaccination of HCMV infected Africans

Autor: Martin R. Goodier, Ed Clarke, Alansana Darboe, Ebrima Danso, Rita Wegmüller, Ebrima S. Touray, Ama Umesi, Eleanor M. Riley, Sophie E. Moore
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Human cytomegalovirus
Male
Diphtheria Toxoid
medicine.medical_treatment
Cytomegalovirus
NK cells
NKG2C
0302 clinical medicine
Tetanus Toxoid
Immunology and Allergy
Interferon gamma
IL-2 receptor
Child
Research Article|Clinical
Influenza vaccine
Research Support
Non-U.S. Gov't
Vaccination
Interleukin-18
Middle Aged
Interleukin-12
3. Good health
Killer Cells
Natural
Poliovirus Vaccines
Cytokine
Influenza Vaccines
Child
Preschool
Cytomegalovirus Infections
Interleukin 12
Female
medicine.drug
Research Article
Trivalent influenza vaccine
Adult
Adolescent
Immunology
Immunity to infection
Immunization
Secondary
Biology
DTPiP vaccine
03 medical and health sciences
Clinical
Interferon-gamma
Young Adult
Antigen
Lysosomal-Associated Membrane Protein 1
Journal Article
medicine
Humans
Vaccines
Combined
Vaccine Potency
Aged
Interleukins
Interleukin-2 Receptor alpha Subunit
medicine.disease
Virology
030104 developmental biology
Africa
030215 immunology
Zdroj: European Journal of Immunology
Darboe, A, Danso, E, Clarke, E, Umesi, A, Touray, E, Wegmuller, R, Moore, S E, Riley, E M & Goodier, M R 2017, ' Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans ', European Journal of Immunology, vol. 47, no. 6, pp. 1040-1050 . https://doi.org/10.1002/eji.201746974
ISSN: 1521-4141
0014-2980
Popis: Human cytomegalovirus (HCMV) infection drives the phenotypic and functional differentiation of NK cells, thereby influencing the responses of these cells after vaccination. NK cell functional differentiation is particularly advanced in African populations with universal exposure to HCMV. To investigate the impact of advanced differentiation on vaccine-induced responses, we studied NK-cell function before and after vaccination with Trivalent Influenza Vaccine (TIV) or diphtheria, tetanus, pertussis, inactivated poliovirus vaccine (DTPiP) in Africans with universal, lifelong HCMV exposure. In contrast to populations with lower prevalence of HCMV infection, no significant enhancement of NK-cell responses (IFN-γ, CD107a, CD25) occurred after in vitro re-stimulation of post-vaccination NK cells with TIV or DTPiP antigens compared to pre-vaccination baseline cells. However, both vaccinations resulted in higher frequencies of NK cells producing IFN-γ in response to exogenous IL-12 with IL-18, which persisted for up to 6 months. Enhanced cytokine responsiveness was restricted to less differentiated NK cells, with increased frequencies of IFN-γ+ cells observed within CD56bright CD57- , CD56dim CD57- NKG2C- and CD56dim CD57- NKG2C+ NK-cell subsets. These data suggest a common mechanism whereby different vaccines enhance NK cell IFN-γ function in HCMV infected donors and raise the potential for further exploitation of NK cell "pre-activation" to improve vaccine effectiveness.
Databáze: OpenAIRE
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