Enhancement of cytokine‐driven NK cell IFN‐γ production after vaccination of HCMV infected Africans
Autor: | Martin R. Goodier, Ed Clarke, Alansana Darboe, Ebrima Danso, Rita Wegmüller, Ebrima S. Touray, Ama Umesi, Eleanor M. Riley, Sophie E. Moore |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Human cytomegalovirus Male Diphtheria Toxoid medicine.medical_treatment Cytomegalovirus NK cells NKG2C 0302 clinical medicine Tetanus Toxoid Immunology and Allergy Interferon gamma IL-2 receptor Child Research Article|Clinical Influenza vaccine Research Support Non-U.S. Gov't Vaccination Interleukin-18 Middle Aged Interleukin-12 3. Good health Killer Cells Natural Poliovirus Vaccines Cytokine Influenza Vaccines Child Preschool Cytomegalovirus Infections Interleukin 12 Female medicine.drug Research Article Trivalent influenza vaccine Adult Adolescent Immunology Immunity to infection Immunization Secondary Biology DTPiP vaccine 03 medical and health sciences Clinical Interferon-gamma Young Adult Antigen Lysosomal-Associated Membrane Protein 1 Journal Article medicine Humans Vaccines Combined Vaccine Potency Aged Interleukins Interleukin-2 Receptor alpha Subunit medicine.disease Virology 030104 developmental biology Africa 030215 immunology |
Zdroj: | European Journal of Immunology Darboe, A, Danso, E, Clarke, E, Umesi, A, Touray, E, Wegmuller, R, Moore, S E, Riley, E M & Goodier, M R 2017, ' Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans ', European Journal of Immunology, vol. 47, no. 6, pp. 1040-1050 . https://doi.org/10.1002/eji.201746974 |
ISSN: | 1521-4141 0014-2980 |
Popis: | Human cytomegalovirus (HCMV) infection drives the phenotypic and functional differentiation of NK cells, thereby influencing the responses of these cells after vaccination. NK cell functional differentiation is particularly advanced in African populations with universal exposure to HCMV. To investigate the impact of advanced differentiation on vaccine-induced responses, we studied NK-cell function before and after vaccination with Trivalent Influenza Vaccine (TIV) or diphtheria, tetanus, pertussis, inactivated poliovirus vaccine (DTPiP) in Africans with universal, lifelong HCMV exposure. In contrast to populations with lower prevalence of HCMV infection, no significant enhancement of NK-cell responses (IFN-γ, CD107a, CD25) occurred after in vitro re-stimulation of post-vaccination NK cells with TIV or DTPiP antigens compared to pre-vaccination baseline cells. However, both vaccinations resulted in higher frequencies of NK cells producing IFN-γ in response to exogenous IL-12 with IL-18, which persisted for up to 6 months. Enhanced cytokine responsiveness was restricted to less differentiated NK cells, with increased frequencies of IFN-γ+ cells observed within CD56bright CD57- , CD56dim CD57- NKG2C- and CD56dim CD57- NKG2C+ NK-cell subsets. These data suggest a common mechanism whereby different vaccines enhance NK cell IFN-γ function in HCMV infected donors and raise the potential for further exploitation of NK cell "pre-activation" to improve vaccine effectiveness. |
Databáze: | OpenAIRE |
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