Enzyme and coenzyme levels, oxygen uptake and lactate production in synchronized cultures of Chinese hamster cells
| Autor: | C.H. Blomquist, Robert A. Tobey, C.T. Gregg |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Pyridines
Coenzymes Mitosis Dehydrogenase Isomerase Glucosephosphate Dehydrogenase Chinese hamster Cofactor Cell Line chemistry.chemical_compound Oxygen Consumption Leucine Cricetinae Culture Techniques Hexokinase Animals chemistry.chemical_classification Carbon Isotopes biology Nucleotides Phosphogluconate Dehydrogenase Glucose-6-Phosphate Isomerase Cell Biology NAD biology.organism_classification Glucose phosphate Molecular biology Glucose Enzyme Phosphoglucomutase chemistry Biochemistry Lactates biology.protein NADP |
| Zdroj: | Experimental Cell Research. 66:75-80 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/s0014-4827(71)80013-7 |
| Popis: | Summary The activities of five enzymes involved in glucose utilization were measured in synchronized cultures of Chinese hamster cells. In the 12 h after mitosis, the activity per cell of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphoglucomutase, and glucose phosphate isomerase increased continuously to 180% of the initial values. The levels of oxidized and reduced pyridine nucleotides increased linearly, the ratio of oxidized-to-reduced forms remained constant, and the rates of oxygen consumption and lactate production were unchanged. These results suggest a stable basal cellular metabolism in these cells throughout interphase. |
| Databáze: | OpenAIRE |
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