Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles
| Autor: | Yulia Zhitnyuk, Noriko Sasakawa, Huaigeng Xu, Mandy S. Y. Lung, Hirohide Saito, Akitsu Hotta, Peter Gee |
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| Rok vydání: | 2018 |
| Předmět: |
Ribosomal Proteins
0301 basic medicine viruses Transgene Biophysics Biochemistry Green fluorescent protein 03 medical and health sciences Transduction (genetics) 0302 clinical medicine Viral Envelope Proteins Humans RNA Messenger Induced pluripotent stem cell Molecular Biology Messenger RNA Membrane Glycoproteins biology Chemistry Gene Transfer Techniques Cell Biology biology.organism_classification Cell biology genomic DNA HEK293 Cells 030104 developmental biology Cell culture Vesicular stomatitis virus 030220 oncology & carcinogenesis |
| Zdroj: | Biochemical and Biophysical Research Communications. 505:1097-1102 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2018.09.113 |
| Popis: | The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this end, we have established a novel mRNA delivery system utilizing chimeric virus-like particles (VLPs). We generated a novel VLP by fusing protein G of Vesicular stomatitis virus (VSV-G) with a ribosomal protein L7Ae of Archeoglobus fulgidus. This system allowed the efficient delivery of EGFP mRNA which was independent from the presence of BoxC/D motif in the mRNA sequence. Our VSVG-L7Ae VLP system demonstrated high transduction efficacy in hard-to-transfect cell lines, such as human induced pluripotent stem cells (iPS cells) and monocytes. In summary, this platform may serve as an efficient and transient transgene delivery tool for an mRNA of interest. |
| Databáze: | OpenAIRE |
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