The Maastricht Strain and England Strain of Rat Cytomegalovirus Represent Different Betaherpesvirus Species Rather Than Strains
| Autor: | Cornelis Vink, Suzanne J.F. Kaptein, Patrick S. Beisser, Cathrien A. Bruggeman, Erik Beuken |
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| Rok vydání: | 1998 |
| Předmět: |
Human cytomegalovirus
Genes Viral Transcription Genetic viruses Molecular Sequence Data Cytomegalovirus Locus (genetics) Biology Immediate-Early Proteins Mice Exon Virology Betaherpesvirinae medicine Animals Humans Antigens Viral Gene Genome size Phylogeny Genetics chemistry.chemical_classification Base Sequence medicine.disease In vitro Rats Amino acid England chemistry DNA Viral RNA splicing |
| Zdroj: | Virology. 246:341-351 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1998.9196 |
| Popis: | The major immediate early (MIE) locus of the Maastricht strain of rat cytomegalovirus (RCMV) was found to comprise five exons of which the first is noncoding. The first three exons are spliced to either exon 4, generating IE1, or exon 5, generating IE2. An additional splicing event unique to RCMV (Maastricht) was identified in exon 5, resulting in a 466-bp deletion. IE1 transcripts were detected exclusively during the IE phase of infectionin vitro,whereas IE2 transcripts were detected during both the IE and late phase of infection. The similarities between amino acid sequences derived from the MIE gene of RCMV (Maastricht) and murine cytomegalovirus are low (22 and 37% for IE1 and IE2, respectively). Surprisingly, the similarities between the MIE proteins of RCMV (Maastricht) and the England strain of RCMV are also low (23 and 32% for IE1 and IE2, respectively). This suggests that these RCMV strains represent different betaherpesvirus species rather than strains. This is underscored by the difference between both viruses in genome size as well as growth characteristics. The existence of two different RCMV-like species might have important implications for the use of these viruses as models for human cytomegalovirus. |
| Databáze: | OpenAIRE |
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