Pre-existing Immunity to Japanese Encephalitis Virus Alters CD4 T Cell Responses to Zika Virus Inactivated Vaccine
Autor: | Michael Koren, Damee Moon, Rafael De La Barrera, Noemia S. Lima, Leyi Lin, Kayvon Modjarrad, Samuel Darko, Richard G. Jarman, Stephen J. Thomas, Nelson L. Michael, Lydie Trautmann, Daniel C. Douek, Kenneth H. Eckels |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine CD4-Positive T-Lymphocytes cross-reactivity T cell viruses 030106 microbiology Immunology Cross Reactions Antibodies Viral Virus Zika virus 03 medical and health sciences Double-Blind Method flavivirus Immunity vaccine medicine Humans Immunology and Allergy Original Research Encephalitis Virus Japanese biology Japanese Encephalitis Vaccines Zika Virus Infection Yellow Fever Vaccine Zika Virus CD4 T cell Japanese encephalitis medicine.disease biology.organism_classification Virology Antibodies Neutralizing Vaccination Flavivirus TCR repertoire 030104 developmental biology medicine.anatomical_structure Vaccines Inactivated Inactivated vaccine Yellow fever virus lcsh:RC581-607 |
Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
ISSN: | 1664-3224 |
DOI: | 10.3389/fimmu.2021.640190 |
Popis: | The epidemic spread of Zika virus (ZIKV), associated with devastating neurologic syndromes, has driven the development of multiple ZIKV vaccines candidates. An effective vaccine should induce ZIKV-specific T cell responses, which are shown to improve the establishment of humoral immunity and contribute to viral clearance. Here we investigated how previous immunization against Japanese encephalitis virus (JEV) and yellow fever virus (YFV) influences T cell responses elicited by a Zika purified-inactivated virus (ZPIV) vaccine. We demonstrate that three doses of ZPIV vaccine elicited robust CD4 T cell responses to ZIKV structural proteins, while ZIKV-specific CD4 T cells in pre-immunized individuals with JEV vaccine, but not YFV vaccine, were more durable and directed predominantly toward conserved epitopes, which elicited Th1 and Th2 cytokine production. In addition, T cell receptor repertoire analysis revealed preferential expansion of cross-reactive clonotypes between JEV and ZIKV, suggesting that pre-existing immunity against JEV may prime the establishment of stronger CD4 T cell responses to ZPIV vaccination. These CD4 T cell responses correlated with titers of ZIKV-neutralizing antibodies in the JEV pre-vaccinated group, but not in flavivirus-naïve or YFV pre-vaccinated individuals, suggesting a stronger contribution of CD4 T cells in the generation of neutralizing antibodies in the context of JEV-ZIKV cross-reactivity. |
Databáze: | OpenAIRE |
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