Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe
| Autor: | Zheng-yun Zhao, Joseph S. Vyle, James H. R. Tucker, John R. Arrand, Jean-Louis H. A. Duprey, Marie San |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Fluorophore Base Pair Mismatch Molecular Sequence Data Clinical Biochemistry Pharmaceutical Science Single-nucleotide polymorphism Polymorphism Single Nucleotide Biochemistry chemistry.chemical_compound Drug Discovery Humans Molecular Biology Gene Fluorescent Dyes Anthracenes Base Sequence Chemistry Hybridization probe Organic Chemistry Temperature Prostatic Neoplasms DNA Molecular biology Models Chemical Duplex (building) Nucleic acid Nucleic Acid Conformation Thermodynamics Molecular Medicine Spectrophotometry Ultraviolet DNA Probes |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 22:129-132 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2011.11.053 |
| Popis: | Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect