The aryloxyalkanoate dioxygenase-12 (AAD-12) protein is not acutely toxic in mice
| Autor: | Johnson Thomas, Rachel M. Golden, Sabitha Papineni |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Food Safety Transgene Food Genetically Modified Pharmacology Biology Pseudomonas fluorescens Toxicology Body weight Dioxygenases Mice 03 medical and health sciences 0404 agricultural biotechnology Bacterial Proteins Dioxygenase High doses Oral route Animals Adverse effect Mice Inbred ICR Herbicides 04 agricultural and veterinary sciences General Medicine Plants Genetically Modified 040401 food science Acute toxicity Genetically modified organism 030104 developmental biology Female Soybeans 2 4-Dichlorophenoxyacetic Acid Food Science |
| Zdroj: | Food and Chemical Toxicology. 110:200-203 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2017.10.036 |
| Popis: | Typically, when a protein is orally toxic, it acts via acute mechanisms, especially at high doses. Therefore, an acute oral toxicity study is considered appropriate for evaluating the safety of transgenic proteins. Soybean plants (events DAS-68416-4 and DAS-444Ø6-6) have been genetically modified to express the aryloxyalkanoate dioxygenase-12 (AAD-12) protein. The AAD-12 protein provides tolerance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). This paper summarizes the study designs of two acute oral toxicity studies evaluating the AAD-12 protein and reports the results of these studies. No mortalities or adverse effects were observed in mice when AAD-12 was tested up to a limit dose of 5000 mg/kg body weight. Based on the results of these studies, it can be concluded that AAD-12 protein, as expressed in genetically modified DAS-68416-4 and DAS-444Ø6-6 soybeans, lacks acute toxicity via the oral route. |
| Databáze: | OpenAIRE |
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