In vitro calcification of chemically functionalized carbon nanotubes

Autor: Romain Mallet, Jack Cousseau, Vincent Fernandez, Michel Félix Baslé, Céline Bergeret, Johanne Beuvelot, Daniel Chappard
Přispěvatelé: Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Thermogravimetric analysis
Materials science
Scanning electron microscope
Carboxylic Acids
Biomedical Engineering
Infrared spectroscopy
02 engineering and technology
Carbon nanotube
Spectrum Analysis
Raman
010402 general chemistry
01 natural sciences
Biochemistry
law.invention
Biomaterials
symbols.namesake
Calcification
Physiologic
Biomimetics
law
Materials Testing
Spectroscopy
Fourier Transform Infrared
Humans
Composite material
Molecular Biology
Tissue Scaffolds
Nanotubes
Carbon
General Medicine
021001 nanoscience & nanotechnology
Body Fluids
0104 chemical sciences
Chemical engineering
Attenuated total reflection
symbols
[PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]
Surface modification
Collagen
Hydroxyapatites
0210 nano-technology
Raman spectroscopy
Biotechnology
Biomineralization
Zdroj: Acta Biomaterialia
Acta Biomaterialia, Elsevier, 2010, 6 (10), pp.4110. ⟨10.1016/j.actbio.2010.05.011⟩
ISSN: 1742-7061
Popis: International audience; Bone is composed of two phases. The organic phase is made of collagen fibrils assembled in broad fibers acting as a template for mineralization. The mineral phase comprises hydroxyapatite (HAP) crystals grown between and inside the collagen fibers. We have developed a biomimetic material using functionalized carbon nanotubes as scaffold to initiate in vitro mineralization. Biomimetic formation of HAP was performed on single-walled carbon nanotubes (SWCNTs) which have been grafted with carboxylic groups. Two types of nanotubes, HiPco(R) and Carbon Solutions(R), were oxidized via various acidic processes, leading to five different groups of carboxylated nanotubes, fully characterized by physical methods (thermogravimetric analysis, attenuated total reflectance infrared spectroscopy and X-ray photoelectron spectroscopy). All samples were dispersed in ultra-pure water and incubated for 2weeks in a synthetic body fluid, in order to induce the calcification of the SWCNTs. Scanning electron microscopy (SEM) and energy-dispersive X-ray analysis studies showed that Ca(2+) and PO(4)(3-) ions were deposited as round-shaped nodules (calcospherites) on the carboxylated SWCNTs. Fourier transform infrared and Raman spectroscopic studies confirmed the HAP formation, and image analysis made on SEM pictures showed that calcospherites and carboxylated SWCNTs were packed together. The size of calcospherites thus obtained in vitro from the HiPco(R) series was close to that issued from calcospherites observed in vivo. Functionalization of SWCNTs with carboxylic groups confers the capacity to induce calcification similar to woven bone.
Databáze: OpenAIRE
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