In vitro calcification of chemically functionalized carbon nanotubes
Autor: | Romain Mallet, Jack Cousseau, Vincent Fernandez, Michel Félix Baslé, Céline Bergeret, Johanne Beuvelot, Daniel Chappard |
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Přispěvatelé: | Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN) |
Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
Thermogravimetric analysis
Materials science Scanning electron microscope Carboxylic Acids Biomedical Engineering Infrared spectroscopy 02 engineering and technology Carbon nanotube Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Biochemistry law.invention Biomaterials symbols.namesake Calcification Physiologic Biomimetics law Materials Testing Spectroscopy Fourier Transform Infrared Humans Composite material Molecular Biology Tissue Scaffolds Nanotubes Carbon General Medicine 021001 nanoscience & nanotechnology Body Fluids 0104 chemical sciences Chemical engineering Attenuated total reflection symbols [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci] Surface modification Collagen Hydroxyapatites 0210 nano-technology Raman spectroscopy Biotechnology Biomineralization |
Zdroj: | Acta Biomaterialia Acta Biomaterialia, Elsevier, 2010, 6 (10), pp.4110. ⟨10.1016/j.actbio.2010.05.011⟩ |
ISSN: | 1742-7061 |
Popis: | International audience; Bone is composed of two phases. The organic phase is made of collagen fibrils assembled in broad fibers acting as a template for mineralization. The mineral phase comprises hydroxyapatite (HAP) crystals grown between and inside the collagen fibers. We have developed a biomimetic material using functionalized carbon nanotubes as scaffold to initiate in vitro mineralization. Biomimetic formation of HAP was performed on single-walled carbon nanotubes (SWCNTs) which have been grafted with carboxylic groups. Two types of nanotubes, HiPco(R) and Carbon Solutions(R), were oxidized via various acidic processes, leading to five different groups of carboxylated nanotubes, fully characterized by physical methods (thermogravimetric analysis, attenuated total reflectance infrared spectroscopy and X-ray photoelectron spectroscopy). All samples were dispersed in ultra-pure water and incubated for 2weeks in a synthetic body fluid, in order to induce the calcification of the SWCNTs. Scanning electron microscopy (SEM) and energy-dispersive X-ray analysis studies showed that Ca(2+) and PO(4)(3-) ions were deposited as round-shaped nodules (calcospherites) on the carboxylated SWCNTs. Fourier transform infrared and Raman spectroscopic studies confirmed the HAP formation, and image analysis made on SEM pictures showed that calcospherites and carboxylated SWCNTs were packed together. The size of calcospherites thus obtained in vitro from the HiPco(R) series was close to that issued from calcospherites observed in vivo. Functionalization of SWCNTs with carboxylic groups confers the capacity to induce calcification similar to woven bone. |
Databáze: | OpenAIRE |
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