Loss of WTAP Impairs Early Parthenogenetic Embryo Development
| Autor: | Dongxu Wang, Jia-Bao Zhang, Yong-Xun Jin, Xianfeng Yu, Mingjun Zhang, Siyi Huang, Jindong Hao |
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| Rok vydání: | 2021 |
| Předmět: |
Homeobox protein NANOG
m6A Veterinary medicine Biology Article 03 medical and health sciences SOX2 SF600-1100 medicine Blastocyst Gene 030304 developmental biology 0303 health sciences Gene knockdown General Veterinary Methyltransferase complex 030302 biochemistry & molecular biology Embryogenesis RNA embryo development parthenogenetic porcine Cell biology WTAP medicine.anatomical_structure QL1-991 Animal Science and Zoology Zoology |
| Zdroj: | Animals, Vol 11, Iss 1675, p 1675 (2021) Animals Volume 11 Issue 6 Animals : an Open Access Journal from MDPI |
| ISSN: | 2076-2615 |
| Popis: | Simple Summary Wilms’ tumor 1-associating protein (WTAP) is a key subunit of the N6-methyl-adenosine (m6A) methyltransferase complex during porcine early embryo development. However, the role of WTAP in embryonic development is still unclear. In this study, we demonstrate that WTAP plays an indispensable role in embryonic development, and the loss of WTAP will promote the apoptosis of embryonic cells, and reduce the rate and quality of embryonic development. Abstract m6A is one of the most common and abundant modifications of RNA molecules present in eukaryotes. The methyltransferase complex, consisting of methyltransferase-like 3 (METTL3), METTL14, and WTAP, is responsible for the m6A modification of RNA. WTAP was identified as an mRNA splicing regulator. Its role as a regulatory subunit of the m6A methyltransferase complex in embryonic development remains largely unknown. To investigate the role of WTAP in porcine early embryonic development, si-WTAP was microinjected into porcine parthenogenetic zygotes. WTAP knockdown significantly reduced the blastocyst rate and global m6A levels, but did not affect the cleavage rate. Betaine was supplemented into the in vitro culture (IVC) to increase the m6A levels. Betaine significantly increased the global m6A levels but did not affect the blastocyst rate. Furthermore, the pluripotency genes, including OCT4, SOX2, and NANOG, were downregulated following WTAP knockdown. The apoptotic genes BAX and CASPASE 3 were upregulated, while the anti-apoptotic gene BCL2 was downregulated in WTAP knockdown blastocysts. TUNEL staining revealed that the number of apoptotic cells was significantly increased following WTAP knockdown. Our study indicated that WTAP has an indispensable role in porcine early embryonic development. |
| Databáze: | OpenAIRE |
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