Serum IgA immune response to individual rotavirus polypeptides in young children with rotavirus infection
| Autor: | G. Stintzing, I. Uhnoo, Kari Johansen, L. Granqvist, K. Karlén, Lennart Svensson |
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| Rok vydání: | 1994 |
| Předmět: |
Diarrhea
Radioimmunoprecipitation Assay viruses Reoviridae Viral Nonstructural Proteins Antibodies Viral medicine.disease_cause Rotavirus Infections Virus Capsid Immune system Lectins Virology Rotavirus medicine Humans Antigens Viral biology Immunogenicity Infant virus diseases General Medicine biology.organism_classification Immunoglobulin A Child Preschool Immunoglobulin G Humoral immunity Immunology biology.protein Jacalin Capsid Proteins Plant Lectins Antibody |
| Zdroj: | Archives of Virology. 138:247-259 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01379129 |
| Popis: | A human IgA-radioimmunoprecipitation assay (IgA-RIPA) utilizing the galactose-binding lectin jacalin from the jack-fruit Artrocarpus integrifolia was developed. Among the human immunoglobulins, jacalin binds specifically to immunoglobulin A. The IgA-RIPA was used to characterize the serum IgA response to individual rotavirus polypeptides in nine paired sera from children (8-34 months of age) with an acute rotavirus infection. In acute sera the IgA response was mainly directed against the inner capsid proteins VP2 and VP6, with VP2 surprisingly being the most immunogenic protein while in the convalescent sera, the IgA response was directed not only against structural but also against non-structural proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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