Proteomic Analysis of Saliva from Patients with Oral Chronic Graft-Versus-Host Disease
| Autor: | Ivana Devic, Lloyd Mancl, Michele E. Lloid, Mark M. Schubert, Jing Zhang, Thomas H. Morton, Min Shi, Melody Missaghi, Richard B. Presland, Catherine Pan, Kenneth T. Izutsu |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Proteomics Saliva medicine.medical_treatment Graft vs Host Disease Hematopoietic stem cell transplantation Mass Spectrometry Article immune system diseases hemic and lymphatic diseases Medicine Humans Oral mucosa Aged Transplantation business.industry Mouth Mucosa Hematology Chronic graft-versus-host disease Middle Aged medicine.disease Acquired immune system 3. Good health Cystatin B Graft-versus-host disease medicine.anatomical_structure Immunology Chronic Disease Female Biomarker discovery business Complication Mouth Diseases iTRAQ mass spectrometry |
| Zdroj: | Biology of Blood and Marrow Transplantation. 20(7):1048-1055 |
| ISSN: | 1083-8791 |
| DOI: | 10.1016/j.bbmt.2014.03.031 |
| Popis: | Chronic graft-versus-host disease (cGVHD) is an immune-mediated disorder and is the major long-term complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The oral mucosa, including the salivary glands, is affected in the majority of patients with cGVHD; however, at present there is only a limited understanding of disease pathobiology. In this study, we performed a quantitative proteomic analysis of saliva pooled from patients with and without oral cGVHD—cGVHD(+) and cGVHD(−), respectively—using isobaric tags for relative and absolute quantification labeling, followed by tandem mass spectrometry. Among 249 salivary proteins identified by tandem mass spectrometry, 82 exhibited altered expression in the oral cGVHD(+) group compared with the cGVHD(−) group. Many of the identified proteins function in innate or acquired immunity, or are associated with tissue maintenance functions, such as proteolysis or the cytoskeleton. Using ELISA immunoassays, we further confirmed that 2 of these proteins, IL-1 receptor antagonist and cystatin B, showed decreased expression in patients with active oral cGVHD (P |
| Databáze: | OpenAIRE |
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