Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure

Autor: Frederik J. Becher, Jérémie Marion, Dominique J. Bicout, Judith Peters, Thomas Heimburg, Marcus Trapp, Thomas Gutberlet
Přispěvatelé: Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Environnement et Prédiction de la Santé des Populations (TIMC-IMAG-EPSP), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Scientific reports 7(1), 15339 (2017). doi:10.1038/s41598-017-15582-4
Peters, J, Marion, J, Becher, F J, Trapp, M, Gutberlet, T, Bicout, D J & Heimburg, T 2017, ' Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure ', Scientific Reports, vol. 7, 15339 . https://doi.org/10.1038/s41598-017-15582-4
Scientific Reports
Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/s41598-017-15582-4⟩
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
'Scientific Reports ', vol: 7, pages: 15339-1-15339-15 (2017)
ISSN: 2045-2322
DOI: 10.1038/s41598-017-15582-4
Popis: We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevant thermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motions of a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed that the volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol% cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry. Neutron backscattering spectroscopy showed that the mean square displacements of H atoms do exhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added at concentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage of these results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccai model equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approach as function of temperature and of pressure, as both are equally important and complementary thermodynamic variables.
Databáze: OpenAIRE
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