A nuclear DNA barcode for eastern North American oaks and application to a study of hybridization in an Arboretum setting
| Autor: | Matthew S. Lobdell, Erwan Guichoux, Elisabeth Fitzek, Adline Delcamp, Andrew L. Hipp, Marlene Hahn |
|---|---|
| Přispěvatelé: | The Morton Arboretum, Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), The Field Museum |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
code barre adn polymorphisme des nucléotides simples Range (biology) [SDV]Life Sciences [q-bio] Introduced species Biology DNA barcoding DNA sequencing 03 medical and health sciences RAD‐seq amérique du nord Genotyping hybridization Ecology Evolution Behavior and Systematics Nature and Landscape Conservation Ecology Phylogenetic tree extraction adn 15. Life on land barcoding SNP barcode Nuclear DNA north American white oaks 030104 developmental biology Sympatric speciation Evolutionary biology quercus |
| Zdroj: | Ecology and Evolution Ecology and Evolution, Wiley Open Access, 2018, 8 (11), pp.5837-5851. ⟨10.1002/ece3.4122⟩ Ecology and Evolution 11 (8), 5837-5851. (2018) |
| ISSN: | 2045-7758 |
| DOI: | 10.1002/ece3.4122⟩ |
| Popis: | International audience; DNA barcoding has proved difficult in a number of woody plant genera, including the ecologically important oak genus Quercus. In this study, we utilized restrictionsite-associated DNA sequencing (RAD-seq) to develop an economical single nucleotide polymorphism (SNP) DNA barcoding system that suffices to distinguish eight common, sympatric eastern North American white oak species. Two de novo clustering pipelines, PyRAD and Stacks, were used in combination with postclustering bioinformatic tools to generate a list of 291 potential SNPs, 80 of which were included in a barcoding toolkit that is easily implemented using MassARRAY mass spectrometry technology. As a proof-of-concept, we used the genotyping toolkit to infer potential hybridization between North American white oaks transplanted outside of their native range (Q.michauxii, Q.montana, Q muehlenbergii/Q.prinoides, and Q.stellata) into a horticultural collection surrounded by natural forests of locally native trees (Q.alba and Q.macrocarpa) in the living collection at The Morton Arboretum (Lisle, IL, USA). Phylogenetic and clustering analyses suggested low rates of hybridization between cultivated and native species, with the exception of one Q.michauxii mother tree, the acorns of which exhibited high admixture from either Q.alba or Q.stellata and Q.macrocarpa, and a hybrid between Q.stellata that appears to have backcrossed almost exclusively to Q.alba. Together, RAD-seq and MassARRAY technologies allow for efficient development and implementation of a multispecies barcode for one of the more challenging forest tree genera. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text