Relatedness of the O-polysaccharide structures of Escherichia coli O123 and Salmonella enterica O58, both containing 4,6-dideoxy-4-{N-[(S)-3-hydroxybutanoyl]-d-alanyl}amino-d-glucose; revision of the E. coli O123 O-polysaccharide structure
| ISSN: | 0008-6215 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d08ca5bae7d79d715faf2f4c6b6d62cc https://doi.org/10.1016/j.carres.2010.01.019 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....d08ca5bae7d79d715faf2f4c6b6d62cc |
| Autor: | Alexander S. Shashkov, Sof'ya N. Senchenkova, Bin Liu, Andrei V. Perepelov, Lei Wang, Yuriy A. Knirel, Lu Feng, Sergei D. Shevelev |
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Spectrometry Mass Electrospray Ionization Magnetic Resonance Spectroscopy Stereochemistry Molecular Sequence Data medicine.disease_cause Polysaccharide Biochemistry Analytical Chemistry chemistry.chemical_compound D-Glucose Escherichia coli medicine Organic chemistry chemistry.chemical_classification biology Polysaccharides Bacterial Organic Chemistry Salmonella enterica Glycoside General Medicine Nuclear magnetic resonance spectroscopy Carbon-13 NMR biology.organism_classification Carbohydrate Sequence chemistry Ethylene glycol |
| Zdroj: | Carbohydrate Research. 345:825-829 |
| ISSN: | 0008-6215 |
| Popis: | O-Polysaccharides were isolated by mild acid degradation of the lipopolysaccharides of Escherichia coli O123 and Salmonella enterica O58 and studied by chemical methods and 2D (1)H and (13)C NMR spectroscopy, including experiments in a H(2)O/D(2)O mixture, which enabled observation of correlations for nitrogen-linked protons. The following structure of the O-polysaccharide of E. coli O123 was established: -->3)-beta-D-Quip4NAlaHb-(1-->6)-alpha-D-GlcpNAc-(1-->3)-alpha-L-QuipNAc-(1-->3)-alpha-D-Glcp (6)(approx. 30% OAc)NAc-(1--> where L-QuipNAc stands for 2-acetamido-2,6-dideoxy-L-glucose and D-Qui4NAlaHb for 4-{N-[(S)-3-hydroxybutanoyl]-D-alanyl}amino-4,6-dideoxy-D-glucose. The latter was isolated as an ethylene glycol glycoside by three sequential Smith degradations of the O-deacetylated O-polysaccharide. The structure established in this work is at variance with the E. coli O123-polysaccharide structure reported earlier [Clark, C. G.; Kropinski, A. M.; Parolis, H.; Grant, C. C.; Trout-Yakel, K. M.; Franklin, K.; Ng, L. K.; Paramonov, N. A.; Parolis, L. A.; Rahn, K.; Tabor, H. J. Med. Microbiol.2009, 58, 884-894]. In accordance with the genetic data, the O-polysaccharide of S. enterica O58 has the same structure, except for it lacks the O-acetylation. |
| Databáze: | OpenAIRE |
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