Two alternative mechanisms for initiation of DNA replication forks in bacteriophage T4: priming by RNA polymerase and by recombination
| Autor: | Gisela Mosig, Andreas Luder |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
DNA Replication
Recombination Genetic Multidisciplinary DNA clamp biology DNA polymerase DNA polymerase II DNA replication RNA-dependent RNA polymerase DNA-Directed RNA Polymerases Virus Replication Models Biological Molecular biology DNA Viral Escherichia coli biology.protein T-Phages Primase DNA polymerase I Polymerase Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 79:1101-1105 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.79.4.1101 |
| Popis: | We show that bacteriophage T4 has two alternative mechanisms to initiate DNA replication; one dependent on Escherichia coli RNA polymerase (RNA nucleotidyltransferase, EC 2.7.7.6), and one dependent on general recombination. Continued DNA synthesis under recombination-defective conditions was sensitive to rifampin, an inhibitor of RNA polymerase. On the other hand, DNA synthesis accelerated in spite of the present of rifampin if recombination occurred. |
| Databáze: | OpenAIRE |
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