Isolation of homogeneous polysaccharide monooxygenases from fungal sources and investigation of their synergism with cellulases when acting on cellulose
| Autor: | A. D. Satrutdinov, A. G. Bulakhov, Arkady P. Sinitsyn, Veronika Yu. Matys, A. V. Koshelev, A. V. Chekushina, Alexander V. Gusakov |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cellobiose dehydrogenase Biophysics Cellulase Polysaccharide 01 natural sciences Biochemistry Biochemistry Genetics and Molecular Biology (miscellaneous) Mixed Function Oxygenases Fungal Proteins 03 medical and health sciences Hydrolysis chemistry.chemical_compound Ascomycota Gallic Acid 010608 biotechnology Cellulases Cellulose Trichoderma reesei chemistry.chemical_classification biology Penicillium General Medicine Monooxygenase biology.organism_classification Recombinant Proteins Kinetics 030104 developmental biology Enzyme chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Carbohydrate Dehydrogenases Electrophoresis Polyacrylamide Gel Geriatrics and Gerontology Peptides |
| Zdroj: | Biochemistry (Moscow). 81:530-537 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297916050102 |
| Popis: | Lytic polysaccharide monooxygenases (PMO) discovered several years ago are enzymes classified as oxidoreductases. In nature, they participate in microbial degradation of cellulose together with cellulases that belong to the hydrolytic type of enzymes (class of hydrolases). Three PMO from ascomycetes - Thielavia terrestris, Trichoderma reesei, and Myceliophthora thermophila - were isolated and purified to homogeneous state using various types of chromatography. The first two enzymes are recombinant proteins heterologously expressed by the Penicillium verruculosum fungus, while the third is a native PMO secreted by M. thermophila. When acting on microcrystalline cellulose, all these PMOs displayed synergism with the cellulase complex of the P. verruculosum fungus. Replacing 10% of cellulases (by protein concentration) with PMO in the presence of 6.25 mM gallic acid or 2.5 µM of cellobiose dehydrogenase from M. thermophila, used as electron donors for PMO, resulted in the 17-31% increase in the yield of reducing sugars after 24-48 h of the enzymatic reaction. |
| Databáze: | OpenAIRE |
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