Three Approaches to the Radioimmunoassay of Human β-Thromboglobulin

Autor:	C.A. Ludlam, S. Moore, A.E. Bolton, J. D. Cash, D. S. Pepper
Rok vydání:	1976
Předmět:	Antiserum Chromatography integumentary system Chemistry Immune Sera Beta-Globulins Radioimmunoassay Hematology Plasma levels Urine Incubation period Dilution Iodine Radioisotopes TRACER Immunology Humans Platelet
Zdroj:	British Journal of Haematology. 33:233-238
ISSN:	1365-2141 0007-1048
DOI:	10.1111/j.1365-2141.1976.tb03534.x
Popis:	Three radioimmunoassays for the measurement of beta-thromboglobulin are described. The standard method, using antiserum in solution, could be used to measure plasma concentrations of beta-thromboglobulin with the results available after 2-3 d. The use of a greater dilution of antiserum and tracer, with delayed addition of tracer, resulted in a more sensitive assay suitable for measuring b-thromboglobulin in urine. The use of a solid-couples antiserum under non-equilibrium conditions allowed the measurement of plasma levels of beta-thromboglobulin after an assay incubation time of 1 h. These three radioimmunoassay systems for beta-thromboglobulin cover the likely clinical requirements for the measurement of this platelet specific protein.
Databáze:	OpenAIRE
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